鉴定和代谢组学分析 Crypthecodinium cohnii 中脂质积累的化学调节剂。
Identification and metabolomic analysis of chemical modulators for lipid accumulation in Crypthecodinium cohnii.
机构信息
Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; SynBio Platform, Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China.
Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; SynBio Platform, Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China.
出版信息
Bioresour Technol. 2015 Sep;191:362-8. doi: 10.1016/j.biortech.2015.03.068. Epub 2015 Mar 18.
In the study, fourteen chemical modulators from five groups (i.e., auxin, gibberellin, cytokinin, signal transducer and amine) were evaluated for their effects on lipid accumulation in Crypthecodinium cohnii. The results showed that naphthoxyacetic acid (BNOA), 2-chlorodracylicacid, salicylic acid (SA), abscisic acid (ABA) and ethanolamine (ETA), increased lipid accumulation in C. cohnii by 10.00-18.78%. In addition, the combined uses of the above chemicals showed that two combinations, 1.0mg/L SA & 152.7 mg/L ETA and 4.0mg/L BNOA & 152.7 mg/L ETA, increased lipid accumulation by 22.45% and 20.54%, respectively. Moreover, a targeted metabolomic approach was employed to decipher the possible mechanisms responsible for the increased lipid accumulation, and the results showed that the enhanced metabolism in glycolysis and TCA cycle as well as the decreased metabolism in PPP pathway could be important for the stimulatory roles of BNOA & ETA and SA & ETA on lipid accumulation in C. cohnii.
在这项研究中，我们评估了来自五个组（即生长素、赤霉素、细胞分裂素、信号转导和胺）的 14 种化学调节剂对 Crypthecodinium cohnii 中脂质积累的影响。结果表明，萘氧基乙酸（BNOA）、2-氯代二羟酸、水杨酸（SA）、脱落酸（ABA）和乙醇胺（ETA）可使 C. cohnii 中的脂质积累增加 10.00-18.78%。此外，上述化学物质的联合使用表明，两种组合，1.0mg/L SA 和 152.7mg/L ETA 以及 4.0mg/L BNOA 和 152.7mg/L ETA，分别使脂质积累增加了 22.45%和 20.54%。此外，我们还采用了靶向代谢组学方法来揭示可能导致脂质积累增加的机制，结果表明，糖酵解和 TCA 循环中的代谢增强以及 PPP 途径中的代谢减弱可能是 BNOA & ETA 和 SA & ETA 对 C. cohnii 中脂质积累的刺激作用的重要原因。
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