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分析与原始栅藻菌株相比，等离子体诱导突变体栅藻菌株中的油脂积累机制。

Analysis of oil accumulation mechanisms in plasma induced mutant Scenedesmus strains compared to original Scenedesmus strains.

机构信息

Shaanxi Institute of Fashion Engineering, Xianyang, 712046, Shaanxi, China.

Xi'an Energy Conservation and Green Development Research Institute Co., Ltd., Xi'an, 710016, Shaanxi, China.

出版信息

Sci Rep. 2024 Sep 11;14(1):21250. doi: 10.1038/s41598-024-72381-4.

DOI:10.1038/s41598-024-72381-4
PMID:39261680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11391082/
Abstract

Scenedesmus sp. is a species of the Scenedesmus genus within the phylum Chlorophyta, commonly found as a planktonic algal species in freshwater and known for its rapid growth rate. This study employs room-temperature, atmospheric-pressure plasma mutagenesis for the breeding of Scenedesmus sp., utilizing transcriptomic analysis to investigate the biosynthesis mechanism of triglycerides. Further analysis of differentially expressed genes in transcriptome by measuring the macroscopic biological indicators of mutant and original algal strains. The findings of the study suggest that the mutant strain's photosynthesis has been enhanced, leading to improved light energy utilization and CO fixation, thereby providing more carbon storage and energy for biomass and lipid production. The intensification of glycolysis and the TCA (tricarboxylic acid) cycle results in a greater shift in carbon flux towards lipid accumulation. An elevated expression level of related enzymes in starch and protein degradation pathways may enhance acetyl CoA accumulation, facilitating a larger substrate supply for fatty acid production and thereby increasing lipid yield.

摘要

钝顶螺旋藻是绿藻门绿藻属的一种，通常作为浮游藻类存在于淡水中，以其快速的生长速度而闻名。本研究采用室温常压等离子体诱变技术对钝顶螺旋藻进行培育，利用转录组分析研究三酰基甘油的生物合成机制。通过测量突变体和原始藻株的宏观生物指标，对转录组中差异表达基因进行进一步分析。研究结果表明，突变株的光合作用得到了增强，从而提高了对光能的利用和 CO 的固定，为生物质和脂质的生产提供了更多的碳储存和能量。糖酵解和三羧酸 (TCA) 循环的强化导致更多的碳通量向脂质积累转移。淀粉和蛋白质降解途径中相关酶的表达水平升高，可能会促进乙酰辅酶 A 的积累，为脂肪酸的产生提供更大的底物供应，从而提高脂质产量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/e5adf10a0c35/41598_2024_72381_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/e5adf10a0c35/41598_2024_72381_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/ac913f5bcc41/41598_2024_72381_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/fe33a43d634e/41598_2024_72381_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/0e2fcbdb861f/41598_2024_72381_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/0362e16470ac/41598_2024_72381_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/0ad9fd322266/41598_2024_72381_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/f7d307f40a05/41598_2024_72381_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/254a8ae04ce4/41598_2024_72381_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/51343ce3a4d0/41598_2024_72381_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/992cdb8b2d55/41598_2024_72381_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4587/11391082/e5adf10a0c35/41598_2024_72381_Fig10_HTML.jpg

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