Liu Bin, Liu Jin, Sun Peipei, Ma Xiaonian, Jiang Yue, Chen Feng
†School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
§Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.
J Agric Food Chem. 2015 Jun 17;63(23):5640-5. doi: 10.1021/acs.jafc.5b01441. Epub 2015 Jun 4.
Sesamol is a strong antioxidant phenolic compound found in sesame seed. It possesses the ability to scavenge intracellular reactive oxygen species (ROS) and to inhibit malic enzyme activity and NADPH supply, resulting possibly in cell proliferation and alteration in the fatty acid composition. In the present study, the effect of sesamol on the growth and accumulation of docosahexaenoic acid (DHA) was investigated in the marine microalga Crypthecodinium cohnii, a prolific producer of DHA. C. cohnii showed a great decrease in the intracellular ROS level with the addition of sesamol. In contrast, the biomass concentration, DHA content (% of total fatty acids), and DHA productivity were significantly increased by 44.20, 11.25, and 20.00%, respectively (P < 0.01). Taken together, this work represents the first report of employing sesamol for enhanced production of DHA by C. cohnii, providing valuable insights into this alga for future biotechnological applications.
芝麻酚是一种存在于芝麻籽中的强抗氧化酚类化合物。它具有清除细胞内活性氧（ROS）以及抑制苹果酸酶活性和NADPH供应的能力，这可能导致细胞增殖和脂肪酸组成的改变。在本研究中，研究了芝麻酚对海洋微藻寇氏隐甲藻（一种高产二十二碳六烯酸（DHA）的藻类）生长和DHA积累的影响。添加芝麻酚后，寇氏隐甲藻细胞内的ROS水平大幅下降。相比之下，生物量浓度、DHA含量（占总脂肪酸的百分比）和DHA生产力分别显著提高了44.20%、11.25%和20.00%（P < 0.01）。综上所述，这项工作首次报道了利用芝麻酚提高寇氏隐甲藻DHA产量，为该藻类未来的生物技术应用提供了有价值的见解。
