Grunow G, Volk H D, Ittenson A
Folia Haematol Int Mag Klin Morphol Blutforsch. 1985;112(1):109-24.
Several methodical aspects for determination of T lymphocytes with Fc receptors for IgM (TM) and IgG (TG) were studied including separation technique of T cells with E-rosetting, culture conditions of T cells for determination of TM and the rosetting of TM and TG with EA complexes. The bests results were obtained by stabilization of E-rosettes with human serum albumin, after separation of E-rosette forming cells lysis of sheep erythrocytes with save hypotonic shock, culturing of T cells in medium containing 20% AB Serum. Furthermore it was shown the possibility using EA complexes produced with not purifieded IgG or IgM anti-ox-red-blood cells antisera without lost of specifity for TM and TG. The percentage of TM and TG in peripheral blood of thirty healthy persons as well as monitoring TM and TG in three cases was investigated.
研究了几种用于测定具有IgM（TM）和IgG（TG）Fc受体的T淋巴细胞的方法学方面，包括用E花环法分离T细胞的技术、用于测定TM的T细胞培养条件以及TM和TG与EA复合物的花环形成。在用人类血清白蛋白稳定E花环、通过安全的低渗休克裂解绵羊红细胞分离E花环形成细胞后、将T细胞培养在含有20%AB血清的培养基中时，获得了最佳结果。此外，还表明使用未纯化的IgG或IgM抗氧红细胞抗血清产生的EA复合物而不丧失对TM和TG的特异性的可能性。研究了30名健康人的外周血中TM和TG的百分比以及3例患者中TM和TG的监测情况。
