Mariani E, Mariani A R, Mazzetti M, Mazzoli M, Baraldini M, Facchini A, Stefanini G F
Boll Soc Ital Biol Sper. 1980 Aug 15;56(15):1590-6.
During the last few years a number of experimental evidences have shown the presence of Fc receptors for IgG or IgM on the membrane of human T cells. These two different receptors are detectable and mutually exclusive on distinct cell populations named respectively TG, TM and T "null" (which lack detectable receptors). Studies on the functional activities of these cells have shown that TM and TG lymphocytes play an antitetical role in regulating B cell response, TM exerting an "helper" activity on the differentiation of B lymphocytes while TG having a "suppressor" one. The aim of this study has been to determine the values of these two subpopulations in a group of twenty control subjects. Our results have shown that TG constitute 10%, whereas TM represent 40% of the total T cells. After EA-G rosetting, the purification of this subpopulation on a density gradient has shown an enrichment of more than 90% in TG cells, while TM contaminate this fraction for less than 4%. The purity of the fraction containing TM has been evaluated using the localization of alpha-naphthyl acetate esterase activity, which has shown that more than 88% of the cells in this fraction are positive for this enzyme.
在过去几年中，大量实验证据表明人T细胞膜上存在IgG或IgM的Fc受体。这两种不同的受体在分别命名为TG、TM和T“无”（缺乏可检测到的受体）的不同细胞群体上可检测到且相互排斥。对这些细胞功能活性的研究表明，TM和TG淋巴细胞在调节B细胞反应中起相反作用，TM对B淋巴细胞的分化发挥“辅助”活性，而TG具有“抑制”活性。本研究的目的是确定一组20名对照受试者中这两个亚群的值。我们的结果表明，TG占总T细胞的10%，而TM占40%。在EA - G花环形成后，通过密度梯度对该亚群进行纯化，结果显示TG细胞的富集率超过90%，而TM在该部分的污染率低于4%。利用α - 萘乙酸酯酶活性的定位评估了含TM部分的纯度，结果表明该部分中超过88%的细胞对该酶呈阳性。
