Wen Congji, Yan Hai, Fu Shibo, Qian Yunliang, Wang Danru, Wang Chen
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai, 200011, People's Republic of China Department of Plastic Surgery, Yancheng First Peoples' Hospital, 16 Yue He Road, People's Republic of China. 224000.
Department of Orthepedics, Nantong Rich Hospital, No. 2000 Lake Avenue, Nantong, 226010, People's Republic of China.
Exp Biol Med (Maywood). 2016 Jul;241(13):1401-9. doi: 10.1177/1535370215576298. Epub 2015 Mar 27.
Adipose-derived stem cells (ASCs) with multilineage potential can be induced into osteoblasts, adipocytes and chondrocytes. ASCs as seed cell are widely used in the field of tissue engineering, but most studies either use autologous cells as the source or an immunodeficient animal as the host. In our present study, we explored the feasibility of applying allogeneic ASCs and demineralized bone matrix (DBM) scaffolds for repairing tubular bone defects without using immunosuppressive therapy. Allogeneic ASCs were expanded and seeded on DBM scaffolds and induced to differentiate along the osteogenic lineage. Eight Sprague-Dawley (SD) rats were used in this study and bilateral critical-sized defects (8 mm) of the ulna were created and divided into two groups: with ASC-DBM constructs or DBM alone. The systemic immune response and the extent of bone healing were evaluated post-operatively. Twenty-four weeks after implantation, digital radiography (DR) testing showed that new bones had formed in the experimental group. By contrast, no bone tissue formation was observed in the control group. This study demonstrated that allogeneic ASCs could promote bone regeneration and repair tubular bone defects combined with DBM by histologically typical bone without systemic immune response.
具有多向分化潜能的脂肪来源干细胞（ASCs）可被诱导分化为成骨细胞、脂肪细胞和软骨细胞。ASCs作为种子细胞在组织工程领域得到广泛应用，但大多数研究要么使用自体细胞作为来源，要么使用免疫缺陷动物作为宿主。在我们目前的研究中，我们探索了应用异体ASCs和脱矿骨基质（DBM）支架修复管状骨缺损而不使用免疫抑制治疗的可行性。将异体ASCs进行扩增并接种于DBM支架上，诱导其沿成骨谱系分化。本研究使用8只Sprague-Dawley（SD）大鼠，制造双侧尺骨临界尺寸缺损（8毫米），并分为两组：植入ASC-DBM构建体组或仅植入DBM组。术后评估全身免疫反应和骨愈合程度。植入24周后，数字X线摄影（DR）检测显示实验组有新骨形成。相比之下，对照组未观察到骨组织形成。本研究表明，异体ASCs与DBM联合应用可促进骨再生并修复管状骨缺损，形成组织学典型的骨，且无全身免疫反应。
