Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China.
Cell Transplant. 2012;21(12):2711-21. doi: 10.3727/096368912X654966. Epub 2012 Sep 7.
The ideal cells for tissue engineering should have the following characteristics: easy obtainment, safety, immune privilege, the capability of self-renewal, and multipotency. Adipose-derived stem cells (ADSCs) are a promising candidate. However, the immunogenicity of allogeneic mesenchymal stem cells limits their long-term benefits. In this study, we introduced human cytomegalovirus US2/US3 gene into the ADSCs to decrease the expression of MHC I protein of ADSCs and reduce the activation of T-cells of the recipient animals. Moreover, the biosafety and biological characteristics of ADSCs transfected with the US2/US3 genes (ADSCs-US2/US3) were similar to normal ADSCs. Then we took ADSCs-US2/US3 to construct a tissue-engineered bone for repairing bone defects in pigs and found that there were no great differences in repair effects or healing time between the allogeneic ADSCs-US2/US3 group and the autologous ADSC group. These results suggest that allogeneic ADSCs-US2/US3 have the advantages of biological safety, low immunogenicity, and effective osteogenesis. Such barely immunogenic ADSCs will be crucial for the success of future tissue-regenerative approaches.
易于获取、安全、免疫特权、自我更新能力和多能性。脂肪来源的干细胞（ADSCs）是一种很有前途的候选细胞。然而，同种异体间充质干细胞的免疫原性限制了其长期益处。在本研究中，我们将人巨细胞病毒 US2/US3 基因导入 ADSCs 中，以降低 ADSCs 中 MHC I 蛋白的表达，减少受体动物 T 细胞的激活。此外，转染 US2/US3 基因的 ADSCs（ADSCs-US2/US3）的生物安全性和生物学特性与正常 ADSCs 相似。然后，我们使用 ADSCs-US2/US3 构建组织工程骨来修复猪的骨缺损，发现同种异体 ADSCs-US2/US3 组与自体 ADSC 组在修复效果和愈合时间上没有明显差异。这些结果表明，同种异体 ADSCs-US2/US3 具有生物安全性、低免疫原性和有效的成骨作用。这种几乎无免疫原性的 ADSCs 将对未来组织再生方法的成功至关重要。
