Che Juanjuan, Yang Yifan, Xiao Jing, Zhao Pengfei, Yan Bo, Dong Shuo, Cao Bangwei
Department of Oncology, Beijing Friendship Hospital, Capital Medical University, #95 Yong An Road, Xicheng District, Beijing, 100050, People's Republic of China.
Tumour Biol. 2015 Aug;36(8):6559-68. doi: 10.1007/s13277-015-3350-1. Epub 2015 Mar 28.
The deregulation of claudin-3 has been reported to correlate with the invasion and metastasis of various cancers, but little is known about its expression level and the prognostic value in squamous cell lung carcinoma (SqCC). The purpose of this study is to determine the expression levels and the prognostic value of claudin-3 in completely resected SqCC tissues, and the potential underlying mechanism. The protein expression of claudin-3, E-cadherin, β-catenin, and vimentin in the tumor tissues from 103 patients with surgically resected SqCC was examined using immunohistochemistry, western blots, as well as semi-quantitative estimation. The claudin-3 protein level was significantly associated with E-cadherin, β-catenin, and vimentin protein expression. A decreased claudin-3 protein level was significantly correlated with TNM stage, lymph node metastasis, and disease recurrence. Similarly, downregulation of E-cadherin was significantly correlated with lymph node metastasis and disease recurrence. Decreased β-catenin expression also had a significant correlation with disease recurrence. Univariate analyses indicated that the T stage, lymph node metastasis, the TNM stage, and the expression of claudin-3, β-catenin, and vimentin were significant predictors for overall survival (OS). Moreover, multivariate analyses demonstrated that the TNM stage and protein levels of claudin-3, β-catenin, and vimentin were independent predictors for OS of SqCC patients. Claudin-3 plays an important role in the epithelial-mesenchymal transition of SqCC and might be used as a potential prognostic factor for SqCC.
据报道，claudin-3的失调与多种癌症的侵袭和转移相关，但关于其在肺鳞状细胞癌（SqCC）中的表达水平及预后价值却知之甚少。本研究旨在确定claudin-3在完全切除的SqCC组织中的表达水平及其预后价值，以及潜在的机制。采用免疫组织化学、蛋白质印迹法以及半定量评估，检测了103例手术切除的SqCC患者肿瘤组织中claudin-3、E-钙黏蛋白、β-连环蛋白和波形蛋白的蛋白表达。claudin-3蛋白水平与E-钙黏蛋白、β-连环蛋白和波形蛋白的蛋白表达显著相关。claudin-3蛋白水平降低与TNM分期、淋巴结转移及疾病复发显著相关。同样，E-钙黏蛋白下调与淋巴结转移和疾病复发显著相关。β-连环蛋白表达降低也与疾病复发显著相关。单因素分析表明，T分期、淋巴结转移、TNM分期以及claudin-3、β-连环蛋白和波形蛋白的表达是总生存期（OS）的显著预测指标。此外，多因素分析表明，TNM分期以及claudin-3、β-连环蛋白和波形蛋白的蛋白水平是SqCC患者OS的独立预测指标。claudin-3在SqCC的上皮-间质转化中起重要作用，可能作为SqCC的潜在预后因素。
