Lu Hongjian, Shen Jiabing, Song Xinjian, Ge Jianbin, Cai Rixin, Dai Aihua, Jiang Zhongli
Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
The Second People's Hospital of Nantong, Nantong, 226002, China.
Cell Mol Neurobiol. 2015 Oct;35(7):921-30. doi: 10.1007/s10571-015-0187-5. Epub 2015 Mar 29.
Pyrroloquinoline quinone (PQQ) has invoked considerable interest because of its presence in foods, antioxidant properties, cofactor of dehydrogenase, and amine oxidase. Protective roles of PQQ in central nervous system diseases, such as experimental stroke and spinal cord injury models have been emerged. However, it is unclear whether intracerebral hemorrhage (ICH), as an acute devastating disease, can also benefit from PQQ in experimental conditions. Herein, we examined the possible effect of PQQ on neuronal functions following ICH in the adult rats. The results showed that rats pretreated with PQQ at 10 mg/kg effectively improved the locomotor functions, alleviated the hematoma volumes, and reduced the expansion of brain edema after ICH. Also, pretreated rats with PQQ obviously reduced the production of reactive oxygen species after ICH, probably due to its antioxidant properties. Further, we found that, Bcl-2/Bax, the important indicator of oxidative stress insult in mitochondria after ICH, exhibited increasing ratio in PQQ-pretreated groups. Moreover, activated caspase-3, the apoptotic executor, showed coincident alleviation in PQQ groups after ICH. Collectively, we speculated that PQQ might be an effective and potential neuroprotectant in clinical therapy for ICH.
吡咯喹啉醌（PQQ）因其存在于食物中、具有抗氧化特性、作为脱氢酶和胺氧化酶的辅因子而引起了广泛关注。PQQ在中枢神经系统疾病（如实验性中风和脊髓损伤模型）中的保护作用已逐渐显现。然而，脑出血（ICH）作为一种急性破坏性疾病，在实验条件下是否也能从PQQ中获益尚不清楚。在此，我们研究了PQQ对成年大鼠脑出血后神经元功能的可能影响。结果表明，以10mg/kg的剂量对大鼠进行PQQ预处理可有效改善其运动功能，减轻血肿体积，并减少脑出血后的脑水肿扩展。此外，经PQQ预处理的大鼠在脑出血后明显减少了活性氧的产生，这可能归因于其抗氧化特性。进一步地，我们发现，脑出血后线粒体氧化应激损伤的重要指标Bcl-2/Bax在PQQ预处理组中的比例有所增加。此外，凋亡执行者活化的半胱天冬酶-3在脑出血后的PQQ组中也呈现出一致的减轻。总体而言，我们推测PQQ可能是脑出血临床治疗中一种有效且有潜力的神经保护剂。
