Nakano Masahiko, Suzuki Hiroshi, Imamura Tadashi, Lau Annette, Lynch Barry
Niigata Research Laboratory, Mitsubishi Gas Chemical Co., Inc., 182 Tayuhama, Shinwari, Kita-ku, Niigata 950-3112, Japan.
Regul Toxicol Pharmacol. 2013 Nov;67(2):189-97. doi: 10.1016/j.yrtph.2013.07.007. Epub 2013 Jul 25.
The genotoxic potential of pyrroloquinoline quinone (PQQ) disodium salt (BioPQQ™) was evaluated in a battery of genotoxicity tests. The results of the bacterial mutation assay (Ames test) were negative. Weak positive results were obtained in 2 separate in vitro chromosomal aberration test in Chinese hamster lung (CHL) fibroblasts. Upon testing in an in vitro chromosomal aberration test in human peripheral blood lymphocytes, no genotoxic activity of PQQ was noted. In the in vivo micronucleus assay in mice, PQQ at doses up to 2,000 mg/kg body weight demonstrated that no genotoxic effects are expressed in vivo in bone marrow erythrocytes. The weak responses in the in vitro test CHL cells were considered of little relevance under conditions of likely human exposure. PQQ disodium was concluded to have no genotoxic activity in vivo.
在一系列遗传毒性试验中评估了吡咯喹啉醌二钠盐（BioPQQ™）的遗传毒性潜力。细菌突变试验（艾姆斯试验）结果为阴性。在中国仓鼠肺（CHL）成纤维细胞进行的2项独立体外染色体畸变试验中获得了弱阳性结果。在人外周血淋巴细胞的体外染色体畸变试验中进行测试时，未发现PQQ的遗传毒性活性。在小鼠体内微核试验中，高达2000mg/kg体重剂量的PQQ表明，在体内骨髓红细胞中未表现出遗传毒性作用。体外CHL细胞试验中的微弱反应在可能的人体暴露条件下被认为相关性不大。得出结论，PQQ二钠在体内无遗传毒性活性。
