Altschuh D, Hartman D, Reinbolt J, Van Regenmortel M H
Mol Immunol. 1983 Mar;20(3):271-8. doi: 10.1016/0161-5890(83)90066-4.
Two antigenic determinants have been located in the vicinity of residues 1-10 and 34-39 of the coat protein of tobacco mosaic virus by means of ELISA and complement fixation inhibition experiments with synthetic peptides. The determinant present in residues 1-10 is also expressed at the outer surface of the subunits when they are assembled into virions. Two other determinants expressed in the dissociated protein subunit have been located in the vicinity of residues 55-61 and 80-90 by means of inhibition tests with tryptic peptides. These results bring to seven the number of antigenic determinants of the protein subunit that have been located.
通过使用合成肽进行酶联免疫吸附测定（ELISA）和补体结合抑制实验，已确定烟草花叶病毒外壳蛋白的两个抗原决定簇位于第1至10位和第34至39位氨基酸残基附近。存在于第1至10位氨基酸残基中的抗原决定簇在亚基组装成病毒粒子时也会在其外表面表达。通过胰蛋白酶肽抑制试验，已在解离的蛋白质亚基中表达的另外两个抗原决定簇位于第55至61位和第80至90位氨基酸残基附近。这些结果使已确定的蛋白质亚基抗原决定簇的数量增加到七个。
