Pappas Harry C, Lovchik Julie A, Whitten David G
†The Nanoscience and Microsystems Engineering Program, University of New Mexico, 1 University of New Mexico, Albuquerque, New Mexico 87131-1341, United States.
‡Center for Biomedical Engineering, Department of Chemical and Biological Engineering, University of New Mexico, 1 University of New Mexico, Albuquerque, New Mexico 87131-1341, United States.
Langmuir. 2015 Apr 21;31(15):4481-9. doi: 10.1021/acs.langmuir.5b00064. Epub 2015 Apr 7.
A wide range of oligo-p-phenylene ethynylenes has been shown to exhibit good biocidal activity against both Gram-negative and Gram-positive bacteria. While cell death may occur in the dark, these biocidal compounds are far more effective in the light as a result of their ability to sensitize the production of cell-damaging reactive oxygen species. In these studies, the interactions of a specific cationic oligo-p-phenylene ethynylene with spore-forming Bacillus atrophaeus and Bacillus anthracis Sterne have been investigated. Flow cytometry assays are used to rapidly monitor cell death as well as spore germination. This compound effectively killed Bacillus anthracis Sterne vegetative cells (over 4 log reduction), presumably by severe perturbations of the bacterial cell wall and cytoplasmic membrane, while also acting as an effective spore germinant in the dark. While 2 log reduction of B. anthracis Sterne spores was observed, it is hypothesized that further killing could be achieved through enhanced germination.
已表明多种聚对苯撑乙炔低聚物对革兰氏阴性菌和革兰氏阳性菌均具有良好的杀菌活性。虽然细胞死亡可能在黑暗中发生，但这些杀菌化合物在光照下效果更佳，因为它们能够敏化产生细胞损伤性活性氧。在这些研究中，已对一种特定的阳离子聚对苯撑乙炔与形成芽孢的萎缩芽孢杆菌和炭疽芽孢杆菌斯特恩菌株的相互作用进行了研究。流式细胞术检测用于快速监测细胞死亡以及芽孢萌发。该化合物有效地杀死了炭疽芽孢杆菌斯特恩营养细胞（减少超过4个对数级），推测是通过严重扰乱细菌细胞壁和细胞质膜，同时在黑暗中还充当有效的芽孢萌发剂。虽然观察到炭疽芽孢杆菌斯特恩芽孢减少了2个对数级，但据推测，通过增强萌发可实现进一步的杀灭。
