抗阻训练通过调节miRNA-214来调控心脏功能。
Resistance training regulates cardiac function through modulation of miRNA-214.
作者信息
Melo Stéphano Freitas Soares, Barauna Valério Garrone, Júnior Miguel Araújo Carneiro, Bozi Luiz Henrique Marchesi, Drummond Lucas Rios, Natali Antônio José, de Oliveira Edilamar Menezes
机构信息
Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil.
Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo, Vitória 29043-900, Brazil.
出版信息
Int J Mol Sci. 2015 Mar 26;16(4):6855-67. doi: 10.3390/ijms16046855.
AIMS
To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes.
MAIN METHODS
Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum.
KEY FINDINGS
RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05).
SIGNIFICANCE
Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression.
目的
确定抗阻训练（RT）对微小RNA（miRNA）-214及其在肌浆网Ca2+ -ATP酶（SERCA2a）中的靶标的表达的影响，以及对分离的左心室心肌细胞的形态和力学特性的影响。
主要方法
将雄性Wistar大鼠分为两组（每组n = 7）：对照组（CO）或训练组（TR）。运动训练方案包括：4×12组，每周5次，共8周，强度为一次最大重复量的80%。
主要发现
与对照动物相比，RT使左心室心肌细胞宽度增加了15%，体积增加了12%（p < 0.05）。与CO组相比，TR组细胞的半松弛时间和峰值时间分别降低了8.4%和4.4%（p < 0.05）。RT使miRNA-214水平降低了18.5%，而其靶标SERCA2a的表达则提高了18.5%（p < 0.05）。
意义
我们的研究结果表明，RT增加了单个左心室心肌细胞的尺寸，还导致细胞收缩和舒张加快。这些力学适应性变化可能与SERCA2a表达增加有关，而SERCA2a表达增加反过来可能与miRNA-214表达降低的表观遗传修饰有关。
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