Yassen Ghaeth H, Sabrah Alaa H A, Eckert George J, Platt Jeffrey A
Division of Dental Biomaterials, Department of Restorative Dentistry, Indiana University School of Medicine, Indianapolis, Indiana.
Division of Dental Biomaterials, Department of Restorative Dentistry, Indiana University School of Medicine, Indianapolis, Indiana; Department of Conservative Dentistry, Faculty of Dentistry, The University of Jordan, Amman, Jordan.
J Endod. 2015 Jun;41(6):956-60. doi: 10.1016/j.joen.2015.02.023. Epub 2015 Mar 29.
We investigated the changes in physiochemical properties of dentin surfaces after performing different endodontic regeneration protocols.
Human dentin slices were randomized into 4 treatment groups and 1 untreated control group (n = 10). One treatment group was irrigated with sodium hypochlorite (NaOCl) for 5 minutes followed by EDTA for 10 minutes. The other 3 treatment groups were irrigated with NaOCl; treated for 4 weeks with triple antibiotic paste (TAP), diluted triple antibiotic paste (DTAP), or calcium hydroxide (Ca[OH]2); and then irrigated with EDTA. After treatment, contact angles between a blood analog and dentin surfaces were evaluated. Surface roughness and chemical composition were characterized using optical profilometry and energy-dispersive X-ray spectroscopy, respectively. One-way analysis of variance followed by Fisher least significant difference tests were used for statistical analyses.
All treatment groups showed a significant reduction in wettability and a significant increase in surface roughness when compared with untreated dentin. Dentin treated with Ca(OH)2 had significantly lower wettability compared with all other groups. No significant difference in wettability was found between dentin treated with DTAP and TAP protocols. Dentin treated with TAP had significantly higher surface roughness compared with all other groups. Untreated dentin and NaOCl + EDTA-treated dentin had significantly higher calcium and phosphorus as well as significantly lower carbon compared with dentin treated with Ca(OH)2, DTAP, and TAP.
Endodontic regeneration protocols had a significant effect on wettability, surface roughness, and chemical composition of surface dentin. The Ca(OH)2 protocol caused a significant reduction in dentin wettability compared with TAP or DTAP protocols.
我们研究了在执行不同的牙髓再生方案后牙本质表面理化性质的变化。
将人牙本质切片随机分为4个治疗组和1个未治疗的对照组（n = 10）。一个治疗组用次氯酸钠（NaOCl）冲洗5分钟，然后用乙二胺四乙酸（EDTA）冲洗10分钟。其他3个治疗组用NaOCl冲洗；用三联抗生素糊剂（TAP）、稀释三联抗生素糊剂（DTAP）或氢氧化钙（Ca[OH]2）治疗4周；然后用EDTA冲洗。治疗后，评估模拟血液与牙本质表面之间的接触角。分别使用光学轮廓仪和能量色散X射线光谱仪对表面粗糙度和化学成分进行表征。采用单因素方差分析，随后进行Fisher最小显著差异检验进行统计分析。
与未治疗的牙本质相比，所有治疗组的润湿性均显著降低，表面粗糙度显著增加。与所有其他组相比，用Ca(OH)2治疗的牙本质润湿性显著更低。在用DTAP和TAP方案治疗的牙本质之间，未发现润湿性有显著差异。与所有其他组相比，用TAP治疗的牙本质表面粗糙度显著更高。与用Ca(OH)2、DTAP和TAP治疗的牙本质相比，未治疗的牙本质和用NaOCl + EDTA治疗的牙本质的钙和磷含量显著更高，碳含量显著更低。
牙髓再生方案对表面牙本质的润湿性、表面粗糙度和化学成分有显著影响。与TAP或DTAP方案相比,Ca(OH)2方案导致牙本质润湿性显著降低。
