使用T4 RNA连接酶对单链DNA/RNA的5'末端进行荧光、同位素或生物素标记。
Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.
作者信息
Kinoshita Y, Nishigaki K, Husimi Y
机构信息
Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa 338, Japan.
出版信息
Nucleic Acids Res. 1997 Sep 15;25(18):3747-8. doi: 10.1093/nar/25.18.3747.
Abstract
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.
摘要
开发了一种单链DNA/RNA的快速5'标记方法，该方法基于在T4 RNA连接酶反应中利用腺苷酸化中间体。该方法通常可用于寡核苷酸和多核苷酸5'端的荧光、同位素或生物素标记。
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本文引用的文献
1
Unexpectedly general replaceability of ATP in ATP-requiring enzymes.
J Biochem. 1997 Jul;122(1):205-11. doi: 10.1093/oxfordjournals.jbchem.a021730.
2
Preparation of oligoribonucleotides containing 4-thiouridine using Fpmp chemistry. Photo-crosslinking to RNA binding proteins using 350 nm irradiation.使用Fpmp化学方法制备含4-硫尿苷的寡核糖核苷酸。利用350纳米辐射对RNA结合蛋白进行光交联。
Nucleic Acids Res. 1996 Aug 15;24(16):3173-80. doi: 10.1093/nar/24.16.3173.
3
Site specific labelling of sugar residues in oligoribonucleotides: reactions of aliphatic isocyanates with 2' amino groups.寡核糖核苷酸中糖残基的位点特异性标记：脂肪族异氰酸酯与2'-氨基的反应
Nucleic Acids Res. 1996 Aug 15;24(16):3129-33. doi: 10.1093/nar/24.16.3129.
4
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.用于合成寡核苷酸5'端标记、亲和纯化和磷酸化的光可裂解生物素亚磷酰胺。
Nucleic Acids Res. 1996 Jan 15;24(2):361-6. doi: 10.1093/nar/24.2.361.
5
Single-stranded DNA ligation by T4 RNA ligase for PCR cloning of 5'-noncoding fragments and coding sequence of a specific gene.利用T4 RNA连接酶进行单链DNA连接，用于特定基因5'非编码片段和编码序列的PCR克隆。
Nucleic Acids Res. 1996 Mar 1;24(5):990-1. doi: 10.1093/nar/24.5.990.
6
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Biotechniques. 1993 Sep;15(3):486-8, 490-2, 494-7.
7
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8
Single-step elongation of oligodeoxynucleotides using terminal deoxynucleotidyl transferase.
Eur J Biochem. 1984 Sep 17;143(3):613-20. doi: 10.1111/j.1432-1033.1984.tb08414.x.
9
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Eur J Biochem. 1984 Jan 2;138(1):125-30. doi: 10.1111/j.1432-1033.1984.tb07890.x.
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