Fujiwara T, Hirai M Y, Chino M, Komeda Y, Naito S
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan.
Plant Physiol. 1992 May;99(1):263-8. doi: 10.1104/pp.99.1.263.
The 7S seed storage protein (beta-conglycinin) of soybean (Glycine max [L]. Merr.) has three major subunits; alpha, alpha', and beta. Accumulation of the beta-subunit, but not the alpha- and alpha'-subunits, has been shown to be repressed by exogenously applied methionine to the immature cotyledon culture system (LP Holowach, JF Thompson, JT Madison [1984] Plant Physiol 74: 576-583) and to be enhanced under sulfate deficiency in soybean plants (KR Gayler, GE Sykes [1985] Plant Physiol 78: 582-585). Transgenic petunia (Petunia hybrida) harboring either the alpha'- or beta-subunit gene were constructed to test whether the patterns of differential expression were retained in petunia. Petunia regulates these genes in a similar way as soybean in response to sulfur nutritional stimuli, i.e. (a) expression of the beta-subunit gene is repressed by exogenous methionine in in vitro cultured seeds, whereas the alpha'-subunit gene expression is not affected; and (b) accumulation of the beta-subunit is enhanced by sulfur deficiency. The pattern of accumulation of major seed storage protein of petunia was not affected by these treatments. These results indicate that this mechanism of gene regulation in response to sulfur nutrition is conserved in petunia even though it is not used to regulate its own major seed storage proteins.
大豆（Glycine max [L]. Merr.）的7S种子贮藏蛋白（β-伴大豆球蛋白）有三个主要亚基：α、α′和β。在未成熟子叶培养系统中，外源施用甲硫氨酸可抑制β亚基的积累，但不影响α和α′亚基的积累（LP Holowach、JF Thompson、JT Madison [1984] Plant Physiol 74: 576 - 583），而在大豆植株缺硫时，β亚基的积累会增强（KR Gayler、GE Sykes [1985] Plant Physiol 78: 582 - 585）。构建了携带α′或β亚基基因的转基因矮牵牛（Petunia hybrida），以测试这种差异表达模式在矮牵牛中是否保留。矮牵牛对硫营养刺激的反应与大豆类似，即：（a）在体外培养的种子中，外源甲硫氨酸可抑制β亚基基因的表达，而α′亚基基因的表达不受影响；（b）缺硫会增强β亚基的积累。这些处理不影响矮牵牛主要种子贮藏蛋白的积累模式。这些结果表明，尽管矮牵牛不利用这种机制来调控自身的主要种子贮藏蛋白，但这种对硫营养的基因调控机制在矮牵牛中是保守的。
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