Instituto de Biología Celular y Neurociencias "Prof. E. De Robertis" (IBCN), CONICET and Departamento de Biología Celular, Histología, Embriología y Genética, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina.
Instituto Cajal-CSIC, Molecular, Cellular and Developmental Neurobiology Department, 28002 Madrid, Spain.
Int J Mol Sci. 2022 Oct 16;23(20):12388. doi: 10.3390/ijms232012388.
Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
克隆细胞分析概述了单个祖细胞的个体发生潜能，允许阐明不同细胞类型及其谱系之间的神经异质性。在这项工作中，我们使用鸡胚作为模型，通过发育来分析视网膜干细胞/祖细胞的潜能。我们在 E3.5（HH21-22）时通过卵内实施 UbC-StarTrack 克隆遗传示踪策略来追踪源自睫状缘单个祖细胞的视网膜细胞谱系。通过鉴定表达相同荧光团组合的同胞细胞，在 E11.5-12（HH38）时的神经视网膜中对衍生细胞后代进行克隆分配。源自睫状缘的细胞后代以柱状聚集的形式分布在周边视网膜，有有限的切线分散。分析表明，在发育的早期阶段，该区域含有多能性和定型祖细胞。
