Kempen Paul J, Greasley Sarah, Parker Kelly A, Campbell Jos L, Chang Huan-Yu, Jones Julian R, Sinclair Robert, Gambhir Sanjiv S, Jokerst Jesse V
1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5427; ; 2. Materials Science & Engineering, Stanford University, Stanford, CA 94305;
4. Department of Materials, Imperial College London, Exhibition Road, London, UK SW7 2AZ.
Theranostics. 2015 Mar 1;5(6):631-42. doi: 10.7150/thno.11389. eCollection 2015.
Increasing cell survival in stem cell therapy is an important challenge for the field of regenerative medicine. Here, we report theranostic mesoporous silica nanoparticles that can increase cell survival through both diagnostic and therapeutic approaches. First, the nanoparticle offers ultrasound and MRI signal to guide implantation into the peri-infarct zone and away from the most necrotic tissue. Second, the nanoparticle serves as a slow release reservoir of insulin-like growth factor (IGF)-a protein shown to increase cell survival. Mesenchymal stem cells labeled with these nanoparticles had detection limits near 9000 cells with no cytotoxicity at the 250 µg/mL concentration required for labeling. We also studied the degradation of the nanoparticles and showed that they clear from cells in approximately 3 weeks. The presence of IGF increased cell survival up to 40% (p<0.05) versus unlabeled cells under in vitro serum-free culture conditions.
提高干细胞治疗中的细胞存活率是再生医学领域面临的一项重要挑战。在此，我们报告了一种诊疗用介孔二氧化硅纳米颗粒，其可通过诊断和治疗方法提高细胞存活率。首先，该纳米颗粒提供超声和磁共振成像信号，以引导其植入梗死周边区域，并远离坏死最严重的组织。其次，该纳米颗粒作为胰岛素样生长因子（IGF）的缓释载体，胰岛素样生长因子是一种已证明可提高细胞存活率的蛋白质。用这些纳米颗粒标记的间充质干细胞在标记所需的250μg/mL浓度下，检测限接近9000个细胞，且无细胞毒性。我们还研究了纳米颗粒的降解情况，并表明它们在大约3周内从细胞中清除。在体外无血清培养条件下，与未标记的细胞相比，IGF的存在使细胞存活率提高了40%（p<0.05）。
