一种改进的用于测定尿总蛋白的邻苯三酚红-钼酸盐法。
An improved pyrogallol red-molybdate method for determining total urinary protein.
作者信息
Orsonneau J L, Douet P, Massoubre C, Lustenberger P, Bernard S
机构信息
Laboratoire de Chimie A--C.H.R. Hôtel-Dieu, Nántes, France.
出版信息
Clin Chem. 1989 Nov;35(11):2233-6.
We adapted the pyrogallol red-molybdate method for total urinary protein to the Cobas Bio centrifugal analyzer. The method is simple, rapid, sensitive, and inexpensive. Addition of 25 mg of sodium dodecyl sulfate per liter to the reagent modifies protein reactivities so that the chromogenicity of human gamma globulins is the same as that of albumin. Results by this method and a comparison method that included gel filtration and a modified biuret reaction correlated well (r = 0.951).
我们将用于检测尿总蛋白的焦性没食子酸红 - 钼酸盐法应用于Cobas Bio离心分析仪。该方法简单、快速、灵敏且成本低廉。在试剂中每升添加25毫克十二烷基硫酸钠可改变蛋白质反应性，使人γ球蛋白的显色性与白蛋白相同。该方法的结果与包括凝胶过滤和改良双缩脲反应的比较方法相关性良好（r = 0.951）。
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