Valle M T, Degl'Innocenti M L, Giordano P, Kunkl A, Costantini M T, Perfumo F, Manca F, Gusmano R
Immunology Unit, S. Martino Hospital, XIII USL, Genoa, Italy.
Clin Nephrol. 1989 Nov;32(5):235-8.
The characterization of cellular populations in peritoneal effluents (PE) of CAPD patients has been the subject of few studies. In order to establish a possible correlation between PE cells and clinical parameters, we studied the immunocompetent cells that are found in uncomplicated patients. In this study we used cytofluorimetric analysis to characterize phenotypically immunocompetent PE cells. We studied 17 children with a mean age of 8.7 +/- 5.3 years, who had been on CAPD for a mean duration of 16 months. The patients never suffered from peritonitis. The effluents were collected after overnight dialysis and centrifuged to concentrate cells. Surface phenotype of PE cells was tested with a panel of monoclonal antibodies. The analysis of 40 PE samples showed that the various cell types were present with different frequencies, suggesting that a dominant phenotype cannot be defined. The ratio between M3 positive cells (monocytic-macrophagic lineage) and T3 positive cells (T lymphocytes) showed a tendency to decrease after initiation of CAPD. T4/T8 ratio in all samples did not differ from that of peripheral lymphocytes. A high frequency of activated T cells (41% +/- 13), defined by the presence of DR antigens on T3+ M3- cells, was seen in PE and confirmed by the high frequency of T cells expressing the IL-2 receptor.
腹膜透析（CAPD）患者腹腔积液（PE）中细胞群的特征鲜有研究。为了确定PE细胞与临床参数之间可能存在的相关性，我们对未出现并发症的患者体内的免疫活性细胞进行了研究。在本研究中，我们采用细胞荧光分析对具有免疫活性的PE细胞进行表型特征分析。我们研究了17名儿童，平均年龄为8.7 +/- 5.3岁，平均接受CAPD治疗16个月。这些患者从未患过腹膜炎。过夜透析后收集流出液并离心浓缩细胞。用一组单克隆抗体检测PE细胞的表面表型。对40份PE样本的分析表明，不同细胞类型以不同频率存在，这表明无法确定占主导地位的表型。M3阳性细胞（单核-巨噬细胞系）与T3阳性细胞（T淋巴细胞）的比例在开始CAPD治疗后呈下降趋势。所有样本中的T4/T8比值与外周淋巴细胞的比值无差异。在PE中观察到高频率的活化T细胞（41% +/- 13），其定义为T3 + M3 - 细胞上存在DR抗原，并通过表达IL - 2受体的T细胞的高频率得到证实。
