Valle M T, Degl'Innocenti M L, Bertelli R, Facchetti P, Perfumo F, Fenoglio D, Kunkl A, Gusmano R, Manca F
Department of Immunology, San Martino Hospital, University of Genoa, Italy.
Clin Exp Immunol. 1995 Jul;101(1):172-6. doi: 10.1111/j.1365-2249.1995.tb02294.x.
Mesothelial cells (MC) from human peritoneal omentum fragments obtained during surgical insertion of peritoneal catheters for continuous peritoneal dialysis in end stage renal failure (ESRF) patients were cultured in vitro. MC exhibited a phenotype different from macrophages, but MHC class II molecules were well expressed. Therefore MC lines were tested for antigen-presenting capacity by pulsing with soluble antigens (tetanus toxoid and purified protein derivative (PPD)) or with a corpusculate antigen (Candida albicans bodies). Autologous peripheral blood mononuclear cells (PBMC) depleted of adherent monocytes and cloned T cells generated from an individual matched for the MHC class II antigen DR2 were used to test antigen-presenting function. MC effectively presented the soluble and corpusculate antigens to autologous and MHC-compatible allogeneic lymphocytes, indicating that they are endowed with both endocytic/phagocytic activity and with processing/presenting capacity. Preincubation of MC with human recombinant interferon-gamma (IFN-gamma) up-regulated MHC class II and intercellular adhesion molecule-1 (ICAM-1) expression, but the effect on antigen-presenting function was not consistent. Since MC are an important component of the peritoneal environment, they may participate, along with macrophages, in activation of specific T cells and in the generation of local cell-mediated immunity to various pathogens.
从终末期肾衰竭（ESRF）患者行持续性腹膜透析腹膜导管置入术时获取的人腹膜大网膜碎片中的间皮细胞（MC）进行体外培养。MC表现出与巨噬细胞不同的表型，但MHC II类分子表达良好。因此，通过用可溶性抗原（破伤风类毒素和纯化蛋白衍生物（PPD））或颗粒性抗原（白色念珠菌菌体）脉冲处理来检测MC系的抗原呈递能力。使用去除贴壁单核细胞的自体外周血单核细胞（PBMC）和从与MHC II类抗原DR2匹配的个体产生的克隆T细胞来测试抗原呈递功能。MC有效地将可溶性和颗粒性抗原呈递给自体和MHC兼容的同种异体淋巴细胞，表明它们具有内吞/吞噬活性以及加工/呈递能力。用人重组干扰素-γ（IFN-γ）对MC进行预孵育会上调MHC II类分子和细胞间黏附分子-1（ICAM-1）的表达，但对抗原呈递功能的影响并不一致。由于MC是腹膜环境的重要组成部分，它们可能与巨噬细胞一起参与特定T细胞的激活以及对各种病原体的局部细胞介导免疫的产生。
