补充维生素D3对维生素D缺乏老年女性炎症指标和氧化应激的影响以及维生素D受体（VDR）基因BsmI多态性的作用：大剂量维生素D3可降低炎症标志物水平。
Effect of vitamin D3 supplementation and influence of BsmI polymorphism of the VDR gene of the inflammatory profile and oxidative stress in elderly women with vitamin D insufficiency: Vitamin D3 megadose reduces inflammatory markers.
作者信息
de Medeiros Cavalcante Isa Gabriela, Silva Alexandre Sérgio, Costa Maria José Carvalho, Persuhn Darlene Camati, Issa Chahira Taha Mahd Ibrahim, de Luna Freire Tiago Lima, da Conceição Rodrigues Gonçalves Maria
机构信息
Graduate Program in Nutrition Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
Graduate Program in Nutrition Sciences, Department of Physical Education, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
出版信息
Exp Gerontol. 2015 Jun;66:10-6. doi: 10.1016/j.exger.2015.03.011. Epub 2015 Mar 28.
OBJECTIVE
This study aimed to evaluate the effect of vitamin D3 megadose supplementation and influence of BsmI polymorphism in the VDR gene on the inflammatory profile and oxidative stress in elderly women with vitamin D deficiency.
METHODS
A double blind, randomized, placebo-controlled trial was conducted with 40 elderly women (aged 68±6 years) diagnosed with vitamin D insufficiency (24.7±3.1 ng/mL). Participants were distributed into a supplementation group that received 200,000 IU of vitamin D3 (SG; n=20) and a placebo group (PG; n=20). Blood samples were collected at baseline and after intervention to analyse the 25(OH)D, parathyroid hormone, serum calcium, ultra-sensitive C-reactive protein (us-CRP), alpha 1-acid glycoprotein (AGP-A), total antioxidant capacity (TAC), and malondialdehyde (MDA) levels, as well as the renal and hepatic function, and genotyping was performed for BsmI polymorphism.
RESULTS
Four weeks after supplementation, elderly women in the SG group showed a significant increase in the serum concentration of 25(OH)D (25.29±2.8 to 31.48±6.0; p=0.0001), which was followed by increased TAC (65.25±15.66 to 71.83±10.71; p=0.03) and decreased serum PTH (46.32±13.2 to 35.45±11.0; p=0.009), us-CRP (0.38±0.3 to 0.19±0.1; p=0.007) and AGP-A levels (75.3±15.4 to 61.1±5.9; p=0.005). Changes in BP, ANAC and MDA were not observed. The 25(OH)D and PTH, us-CRP and AGP-A levels of participants with the BB/Bb genotype were more responsive to supplementation, but their other markers did not change.
CONCLUSIONS
Supplementation with a vitamin D3 megadose reduced inflammatory markers and increased the total antioxidant capacity in elderly women with vitamin D insufficiency. The 25(OH)D, PTH, us-CRP and AGP-A levels of elderly patients with the BB/Bb genotype were more responsive to supplementation compared with those with the bb genotype.
目的
本研究旨在评估大剂量补充维生素D3以及维生素D受体（VDR）基因中BsmI多态性对维生素D缺乏老年女性炎症指标和氧化应激的影响。
方法
对40名诊断为维生素D不足（24.7±3.1 ng/mL）的老年女性（年龄68±6岁）进行了一项双盲、随机、安慰剂对照试验。参与者被分为补充组（SG，n=20），接受200,000 IU维生素D3，和安慰剂组（PG，n=20）。在基线和干预后采集血样，分析25(OH)D、甲状旁腺激素、血清钙、超敏C反应蛋白（us-CRP）、α1-酸性糖蛋白（AGP-A）、总抗氧化能力（TAC）和丙二醛（MDA）水平，以及肾功能和肝功能，并对BsmI多态性进行基因分型。
结果
补充四周后，SG组老年女性血清25(OH)D浓度显著升高（从25.29±2.8至31.48±6.0；p=0.0001），随后TAC升高（从65.25±15.66至71.83±10.71；p=0.03），血清甲状旁腺激素（PTH）降低（从46.32±13.2至35.45±11.0；p=0.009），us-CRP降低（从0.38±0.3至0.19±0.1；p=0.007），AGP-A水平降低（从75.3±15.4至61.1±5.9；p=0.005）。未观察到血压、ANAC和MDA的变化。BB/Bb基因型参与者的25(OH)D、PTH、us-CRP和AGP-A水平对补充更敏感，但其他指标未改变。
结论
大剂量补充维生素D3可降低维生素D不足老年女性的炎症标志物水平并提高总抗氧化能力。与bb基因型老年患者相比，BB/Bb基因型老年患者的25(OH)D、PTH、us-CRP和AGP-A水平对补充更敏感。
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