Weissman Benjamin P, Li Nan-Sheng, York Darrin, Harris Michael, Piccirilli Joseph A
Department of Chemistry, University of Chicago, Chicago, IL, USA.
Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA.
Biochim Biophys Acta. 2015 Nov;1854(11):1737-45. doi: 10.1016/j.bbapap.2015.03.007. Epub 2015 Mar 27.
Experimental analysis of kinetic isotope effects represents an extremely powerful approach for gaining information about the transition state structure of complex reactions not available through other methodologies. The implementation of this approach to the study of nucleic acid chemistry requires the synthesis of nucleobases and nucleotides enriched for heavy isotopes at specific positions. In this review, we highlight current approaches to the synthesis of nucleic acids enriched site specifically for heavy oxygen and nitrogen and their application in heavy atom isotope effect studies. This article is part of a special issue titled: Enzyme Transition States from Theory and Experiment.
动力学同位素效应的实验分析是一种极为强大的方法，可用于获取有关复杂反应过渡态结构的信息，而这些信息是通过其他方法无法获得的。将这种方法应用于核酸化学研究需要合成在特定位置富集重同位素的核碱基和核苷酸。在这篇综述中，我们重点介绍了目前位点特异性富集重氧和重氮的核酸合成方法及其在重原子同位素效应研究中的应用。本文是名为《理论与实验中的酶过渡态》的特刊的一部分。
