定向光学相干断层扫描可提供准确的外核层和亨利纤维层测量结果。
DIRECTIONAL OPTICAL COHERENCE TOMOGRAPHY PROVIDES ACCURATE OUTER NUCLEAR LAYER AND HENLE FIBER LAYER MEASUREMENTS.
作者信息
Lujan Brandon J, Roorda Austin, Croskrey Jason A, Dubis Adam M, Cooper Robert F, Bayabo Jan-Kristine, Duncan Jacque L, Antony Bhavna J, Carroll Joseph
机构信息
*Vision Science Graduate Group, School of Optometry, University of California, Berkeley, Berkeley, California; †West Coast Retina Medical Group, San Francisco, California; ‡Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, Wisconsin; §Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin; ¶Department of Biomedical Engineering, Marquette University, Milwaukee, Wisconsin; and **Department of Ophthalmology, University of California, San Francisco, California.
出版信息
Retina. 2015 Aug;35(8):1511-20. doi: 10.1097/IAE.0000000000000527.
PURPOSE
The outer nuclear layer (ONL) contains photoreceptor nuclei, and its thickness is an important biomarker for retinal degenerations. Accurate ONL thickness measurements are obscured in standard optical coherence tomography (OCT) images because of Henle fiber layer (HFL). Improved differentiation of the ONL and HFL boundary is made possible by using directional OCT, a method that purposefully varies the pupil entrance position of the OCT beam.
METHODS
Fifty-seven normal eyes were imaged using multiple pupil entry positions with a commercial spectral domain OCT system. Cross-sectional image sets were registered to each other and segmented at the top of HFL, the border of HFL and the ONL and at the external limiting membrane. Thicknesses of the ONL and HFL were measured and analyzed.
RESULTS
The true ONL and HFL thicknesses varied substantially by eccentricity and between individuals. The true macular ONL thickness comprised an average of 54.6% of measurements that also included HFL. The ONL and HFL thicknesses at specific retinal eccentricities were poorly correlated.
CONCLUSION
Accurate ONL and HFL thickness measurements are made possible by the optical contrast of directional OCT. Distinguishing these individual layers can improve clinical trial endpoints and assessment of disease progression.
目的
外核层（ONL）包含光感受器细胞核，其厚度是视网膜变性的重要生物标志物。由于存在Henle纤维层（HFL），在标准光学相干断层扫描（OCT）图像中，外核层厚度的准确测量受到干扰。使用定向OCT可以改善外核层与Henle纤维层边界的区分，定向OCT是一种有目的地改变OCT光束瞳孔入射位置的方法。
方法
使用商用光谱域OCT系统，通过多个瞳孔入射位置对57只正常眼睛进行成像。将横断面图像集相互配准，并在Henle纤维层顶部、Henle纤维层与外核层边界以及外界膜处进行分割。测量并分析外核层和Henle纤维层的厚度。
结果
外核层和Henle纤维层的真实厚度在不同偏心率以及个体之间存在很大差异。黄斑区外核层的真实厚度平均占包含Henle纤维层在内测量值的54.6%。特定视网膜偏心率下外核层和Henle纤维层的厚度相关性较差。
结论
定向OCT的光学对比度能够实现外核层和Henle纤维层厚度的准确测量。区分这些单独的层可以改善临床试验终点以及疾病进展评估。
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