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香精油对金诺(柑橘×美味柑橘)柑橘主要采后病原菌的体外和体内活性

In vitro and in vivo activity of essential oils against major postharvest pathogens of Kinnow (Citrus nobilis × C. deliciosa) mandarin.

作者信息

Jhalegar Md Jameel, Sharma R R, Singh Dinesh

机构信息

Division of Post Harvest Technology, Indian Agricultural Research Institute, New Delhi, 110 012 India.

Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012 India.

出版信息

J Food Sci Technol. 2015 Apr;52(4):2229-37. doi: 10.1007/s13197-014-1281-2. Epub 2014 Feb 22.

Abstract

The present study envisages the influence of essential oils namely lemon grass, eucalyptus, clove and neem on Kinnow mandarin with the objective to combat major post harvest diseases and to prolong its availability for longer time in the season. For this, in vitro and in vivo studies were conducted. Poisoned food technique was used for in vitro studies, and for in vivo studies, Kinnow fruit were pre-inoculated with pathogens (Penicillium digitatum and P. italicum), treated with different essential oils and then stored at 5 °C ±1 °C temperature and 85-90 % RH). Our results indicated that all essential oils inhibited the growth (colony diameter) of both pathogens over untreated PDA plates, but the inhibition was the strongest by lemon grass oil. Similarly, under in vivo conditions, all essential oils influenced decay incidence, decay loss, lesion diameter, respiration rate, ethylene evolution, overall acceptability and physiological loss in weight but lemon grass was the most effective. And also the incidence of Penicillium italicum was more noticed in fruits than P. digitatum, however, it was reverse under in vitro conditions. The decay rot at all stages of storage was less in EOs treated fruits than untreated fruits, thereby increasing their storage life significantly. Thus, it is evident from our studies that essential oils have the potential to control green and blue mold without causing any injury or harmful effects on Kinnow mandarin, and EOs can be recommended as a safe method for extending its storage life while maintaining fruit quality.

摘要

本研究旨在探讨柠檬草、桉树、丁香和印楝等精油对金诺橘的影响,目的是对抗主要的采后病害,并在季节中延长其可供应时间。为此,进行了体外和体内研究。体外研究采用毒饵技术,体内研究则先将金诺橘果实接种病原菌(指状青霉和意大利青霉),再用不同的精油处理,然后在5℃±1℃温度和85-90%相对湿度下储存。我们的结果表明,与未处理的PDA平板相比,所有精油均抑制了两种病原菌的生长(菌落直径),但柠檬草油的抑制作用最强。同样,在体内条件下,所有精油都对腐烂发生率、腐烂损失、病斑直径、呼吸速率、乙烯释放、总体可接受性和重量生理损失有影响,但柠檬草最为有效。此外,意大利青霉在果实中的发生率比指状青霉更高,然而,在体外条件下情况则相反。在储存的各个阶段,经精油处理的果实腐烂情况均少于未处理的果实,从而显著延长了它们的储存寿命。因此,从我们的研究中可以明显看出,精油有潜力控制绿霉和蓝霉,而不会对金诺橘造成任何损伤或有害影响,并且精油可被推荐为一种在保持果实品质的同时延长其储存寿命的安全方法。

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