Gul Parveen, Bakht Jehan
Department of Microbiology, Quiad-E-Azam University, Islamabad, Pakistan.
Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, KPK, Pakistan.
J Food Sci Technol. 2015 Apr;52(4):2272-9. doi: 10.1007/s13197-013-1195-4. Epub 2013 Nov 8.
The present study investigates the antimicrobial and preservative potentials of turmeric extracts for food industry. Turmeric extracts prepared in n-hexane, water, chloroform and ethanol were applied to meals as preservatives and antibacterial agent. The samples were assessed microbiologically (total bacterial, total fungal and total coliform counts) and organoleptically (color, odor, taste) at day zero and after 15 days intervals. Meals autoclaved for shorter time (5 min) and treated with combination of 1 % or 2 % turmeric extract preserved for longer period. These results were comparable with samples autoclaved for longer period (15 min) with out turmeric extract. The antibacterial activities of different turmeric extracts were also tested against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Candida albicans by disc diffusion method. Water extracted samples of turmeric stored at room temperature inhibited the growth of Escherichia coli and Salmonella typhi while aqueous extract autoclaved at 121 °C for 30 min reduced the growth of Escherichia coli and Staphylococcus aureus. Methanol extracted samples stored at room temperature or autoclaved at 121 °C was effective to control the growth of all microbes under study. Chloroform and n-hexane extracts (stored at room temperature) showed weak activity against all tested microbes.
本研究调查了姜黄提取物在食品工业中的抗菌和防腐潜力。将用正己烷、水、氯仿和乙醇制备的姜黄提取物作为防腐剂和抗菌剂应用于食品中。在第0天以及每隔15天对样品进行微生物学评估(总细菌数、总真菌数和总大肠菌群数)和感官评估(颜色、气味、味道)。较短时间(5分钟)高压灭菌并经1%或2%姜黄提取物组合处理的食品保存时间更长。这些结果与未经姜黄提取物、较长时间(15分钟)高压灭菌的样品相当。还通过纸片扩散法测试了不同姜黄提取物对大肠杆菌、金黄色葡萄球菌、伤寒沙门氏菌和白色念珠菌的抗菌活性。室温保存的姜黄水提取物抑制了大肠杆菌和伤寒沙门氏菌的生长,而在121°C高压灭菌30分钟的水提取物减少了大肠杆菌和金黄色葡萄球菌的生长。室温保存或在121°C高压灭菌的甲醇提取物对所有受试微生物的生长控制有效。氯仿和正己烷提取物(室温保存)对所有测试微生物的活性较弱。