Kelley K A
Institute for Arthritis and Autoimmunity, Miles Research Center, West Haven, Connecticut 06516.
Cytometry. 1989 Nov;10(6):796-800. doi: 10.1002/cyto.990100618.
A flow cytometer was equipped with a modified sample station to facilitate on-line addition of mediators to the sample and reduce the time of delivery of the sample to the interrogation point. The ready availability of materials and straightforward nature of this design make modifications simple and facilitate measurements of cellular activation. Parameters such as pH, membrane fluidity, and calcium mobilization are easily measured in this system, because detection can be made less than 4 s after addition of mediator with no interruption of sample flow. The sample station modification is described in detail along with methods for mixing and temperature control.
流式细胞仪配备了一个经过改良的样品站，以方便向样品中在线添加介质，并减少将样品输送到检测点的时间。这种设计材料现成且操作简单，使得修改很容易，并且便于测量细胞活化情况。在该系统中，诸如pH值、膜流动性和钙动员等参数很容易测量，因为在添加介质后不到4秒即可进行检测，且不会中断样品流动。本文详细描述了样品站的改良以及混合和温度控制方法。
