Ragasa Consolacion Y, Cabrera Esperanza C, Torres Oscar B, Buluran Adiel Inah, Espineli Dinah L, Raga Dennis D, Shen Chien-Chang
Department of Chemistry, De La Salle University Science and Technology Complex Leandro V. Locsin Campus, Biñan City, Laguna 4024, Philippines ; Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines.
Department of Biology, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines.
Pharmacognosy Res. 2015 Apr-Jun;7(2):138-47. doi: 10.4103/0974-8490.150520.
To isolate the secondary metabolites from the dichloromethane (DCM) extracts of Glinus oppositifolius; to test for the cytotoxicity of a new triterpene, oppositifolone (1); and to test for the hypoglycemic, analgesic, and antimicrobial potentials of 1, DCM and aqueous leaf extracts of G. oppositifolius.
The compounds were isolated by silica gel chromatography and identified by nuclear magnetic resonance spectroscopy. The cytotoxicity potential of 1 was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Triterpene 1, DCM, and aqueous leaf extracts were tested for hypoglycemic potential using the oral glucose tolerance test; analgesic potential using the tail-flick assay, and antimicrobial potential using the disc diffusion method.
The DCM extracts of G. oppositifolius afforded 1, squalene, spinasterol, oleanolic acid, phytol, and lutein from the leaves; squalene and spergulagenin A from the stems; and spinasterol from the roots. Triterpene 1 was cytotoxic against human colon carcinoma 116 with an IC50 value of 28.7 but did not exhibit cytotoxicity against A549. The aqueous leaf extract at 200 mg/kg body weight (BW) exhibited hypoglycemic activity with a pronounced % blood glucose reduction of 70.76% ±17.4% within 0.5 h after introduction. The DCM leaf extract showed a lower % blood glucose reduction of 18.52% ±13.5% at 200 mg/kg BW within 1.5 h after introduction, while 1 did not exhibit hypoglycemic activity. The samples did not exhibit analgesic property and were inactive against multiple drug resistant bacterial pathogens.
The compounds responsible for the hypoglycemic activity of G. oppositifolius which are fast acting (0.5 h) are found in the aqueous leaf extract.
从对叶金午时花的二氯甲烷（DCM）提取物中分离次生代谢产物；测试一种新的三萜类化合物对叶金午时花素（1）的细胞毒性；并测试对叶金午时花素1、DCM和叶水提取物的降血糖、镇痛和抗菌潜力。
通过硅胶柱色谱法分离化合物，并通过核磁共振光谱法进行鉴定。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法测试1的细胞毒性潜力。使用口服葡萄糖耐量试验测试三萜类化合物1、DCM和叶水提取物的降血糖潜力；使用甩尾试验测试镇痛潜力，使用纸片扩散法测试抗菌潜力。
对叶金午时花的DCM提取物从叶中得到了对叶金午时花素1、角鲨烯、菠菜甾醇、齐墩果酸、叶绿醇和叶黄素；从茎中得到了角鲨烯和 Spergulagenin A；从根中得到了菠菜甾醇。三萜类化合物1对人结肠癌细胞116具有细胞毒性，IC50值为28.7，但对A549没有细胞毒性。体重200mg/kg的叶水提取物在引入后0.5小时内表现出降血糖活性，血糖明显降低70.76%±17.4%。体重200mg/kg的DCM叶提取物在引入后1.5小时内血糖降低率较低，为18.52%±13.5%，而对叶金午时花素1没有降血糖活性。这些样品没有镇痛特性，对多重耐药细菌病原体无活性。
对叶金午时花具有快速起效（0.5小时）的降血糖活性的化合物存在于叶水提取物中。
