桑叶多糖调节小鼠骨髓来源的树突状细胞成熟。
Mulberry leaf polysaccharides modulate murine bone-marrow-derived dendritic cell maturation.
作者信息
Xue Ming, Sun Haiyan, Cao Yan, Wang Guangchuan, Meng Yiming, Wang Dongmei, Hong Yansong
机构信息
a Department of Endodontics ; School of Stomatology; China Medical University ; Shenyang , China.
出版信息
Hum Vaccin Immunother. 2015;11(4):946-50. doi: 10.1080/21645515.2015.1011977.
Various components of mulberry leaves, such as iminosugars, flavonoids and polysaccharides, have been reported to exert anti-diabetic activity. The purpose of our present study was to examine the modulating effect of mulberry leaf polysaccharides (MLPs) on murine bone-marrow-derived dendritic cells (BMDCs). The ultrastructure, phenotype and functional maturation of BMDCs were studied using transmission electron microscopy (TEM), flow cytometry (FCM), and tested for phagocytosis, acid phosphatase (ACP) activity using an enzyme linked immunosorbent assay (ELISA). Our results demonstrated that MLPs could markedly induce BMDC maturation by up-regulating the expression of membrane phenotypic markers, such as CD80, CD86, CD83,CD40, and MHC II, down-regulating phagocytosis and ACP activity, and by enhancing the production of interleukin 12 (IL-12) and tumor necrosis factor α (TNF-α) secreted by BMDCs. We therefore concluded that MLPs can positively modulate BMDCs.
据报道，桑叶的各种成分，如亚氨基糖、黄酮类化合物和多糖，具有抗糖尿病活性。我们目前研究的目的是研究桑叶多糖（MLPs）对小鼠骨髓来源的树突状细胞（BMDCs）的调节作用。使用透射电子显微镜（TEM）、流式细胞术（FCM）研究了BMDCs的超微结构、表型和功能成熟，并通过酶联免疫吸附测定（ELISA）检测了吞噬作用、酸性磷酸酶（ACP）活性。我们的结果表明，MLPs可以通过上调膜表型标志物如CD80、CD86、CD83、CD40和MHC II的表达，下调吞噬作用和ACP活性，以及增强BMDCs分泌的白细胞介素12（IL-12）和肿瘤坏死因子α（TNF-α）的产生，显著诱导BMDCs成熟。因此，我们得出结论，MLPs可以正向调节BMDCs。
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