Department of Immunology, School of Basic Medical Science, China Medical University, No. 92, North Second Road, Heping District, Shenyang 110001, PR China.
Int J Biol Macromol. 2013 Mar;54:136-43. doi: 10.1016/j.ijbiomac.2012.12.005. Epub 2012 Dec 12.
Purified Rehmannia glutinosa polysaccharide (RGP) is used as functional foods for the prevention and treatment of various diseases. In this study, we examined the effects of RGP on phenotypic and functional maturation of murine bone marrow derived Dendritic cells (BMDCs). Phenotypic maturation of BMDCs was confirmed by conventional scanning electron microscopy (SEM), flow cytometry (FCM) and functional maturation by transmission electron microscopy (TEM), cytochemistry assay, Acid phosphatase (ACP) activity, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA).We found that RGP up-regulated the expression of CD40, CD80, CD83, CD86 and MHC II molecules of BMDCs, down-regulated pinocytosis and phagocytosis activity, induced IL-12 and TNF-α production of BMDCs. It is therefore concluded that RGP can effectively promote the maturation of DCs. Our study provides evidence and rationale on using RGP in various clinical conditions to enhance host immunity and suggests RGP as a potent adjuvant for the design of DC-based vaccines.
纯化地黄多糖（RGP）被用作预防和治疗各种疾病的功能性食品。在这项研究中，我们研究了 RGP 对鼠骨髓来源树突状细胞（BMDC）表型和功能成熟的影响。通过常规扫描电子显微镜（SEM）、流式细胞术（FCM）证实 BMDC 的表型成熟，通过透射电子显微镜（TEM）、细胞化学测定、酸性磷酸酶（ACP）活性、FITC-葡聚糖、生物测定和酶联免疫吸附测定（ELISA）证实功能成熟。我们发现 RGP 上调了 BMDCs 的 CD40、CD80、CD83、CD86 和 MHC II 分子的表达，下调了胞饮和吞噬作用活性，诱导了 BMDCs 的 IL-12 和 TNF-α的产生。因此，可以得出结论，RGP 可以有效促进 DC 的成熟。我们的研究为在各种临床情况下使用 RGP 来增强宿主免疫力提供了证据和依据，并表明 RGP 可作为设计基于 DC 的疫苗的有效佐剂。
