Borrasca Albanir Gabriel, Aranega Alessandra Marcondes, Filho Osvaldo Magro, Timóteo Carlos Alberto
Int J Oral Maxillofac Implants. 2015 Mar-Apr;30(2):442-9. doi: 10.11607/jomi.3568.
The aim of this study was to evaluate the behavior of a demineralized bone matrix membrane and a polytetrafluoroethylene (PTFE) membrane in the process of bone repair of surgical defects filled with autogenous bone in rats.
Sixty rats weighing approximately 250 g each were selected and separated into three groups: control group without membrane, demineralized bone matrix membrane group, and PTFE membrane group. Bilaterally, surgical defects of 2 mm in diameter were prepared in the tibiae. The defect in the left tibia was filled with particulate autogenous bone collected during the creation of the two defects and was left uncovered (control) or was covered with the membranes investigated by the present study. At 10 or 60 days postoperatively, the rats were euthanized and the left tibiae were submitted to routine laboratory processing for histomorphometric analysis. All groups were evaluated separately on the 10th and 60th days after surgery. The Kruskal-Wallis test was used for group comparison.
The membrane-treated defects showed a delay in healing. Sites treated with demineralized bone matrix membrane showed, as early as day 10, more newly formed bone and slow replacement until day 60. At day 60, the sites covered with demineralized bone matrix membrane and with synthetic membrane showed statistically significant results.
The demineralized bone matrix membrane proved to be biocompatible. In terms of newly formed bone area, both membranes showed similar performance.
本研究旨在评估脱矿骨基质膜和聚四氟乙烯（PTFE）膜在大鼠自体骨填充手术缺损的骨修复过程中的行为。
选取60只体重约250 g的大鼠，分为三组：无膜对照组、脱矿骨基质膜组和PTFE膜组。在双侧胫骨制备直径2 mm的手术缺损。左胫骨缺损用在制造两个缺损过程中收集的颗粒状自体骨填充，不覆盖任何东西（对照）或用本研究调查的膜覆盖。术后10天或60天，对大鼠实施安乐死，将左胫骨进行常规实验室处理以进行组织形态计量分析。所有组在术后第10天和第60天分别进行评估。采用Kruskal-Wallis检验进行组间比较。
用膜处理的缺损愈合延迟。用脱矿骨基质膜处理的部位早在第10天就显示出更多新形成的骨，并且直到第60天替换缓慢。在第60天，用脱矿骨基质膜和合成膜覆盖的部位显示出具有统计学意义的结果。
脱矿骨基质膜被证明具有生物相容性。就新形成的骨面积而言，两种膜表现出相似的性能。
