[Autotransfusion of cord blood erythrocytes in newborns with malformations requiring early surgical intervention].

PURPOSE

To evaluate the clinical efficacy of cord blood erythrocytes autotransfiusion for the correction of anemia in the intra-and postoperative periods in infants requiring early surgical correction of congenital malformations.

METHODS

Washed autoemythrocytes from placental umbilical blood were transfused for correction of intra and post-operative anemias. Umbilical blood assembly was carried out after extraction of the child and navel intersection by the occluded mean by a vein puncture distal (placental) end of a navel by the drainage needle which is a part of special transfiusion system. Further blood in the marked containers was pitched in branch of gravitational surgery of blood where its centrifiugal separation on erythrocyte mass and plasma was made. Then concentrated red cells it was put on storage for 21 day in a cooler at temperature 40 °C. Directly ahead of autotransfusion concentrated red cells was exposed to washing out in sterile physiological solution and a filtration through the micromodular filter. Then the marked package with the washed erythrocytes was pitched in branch of surgery of newborns for the purpose offurther anttqtransfusion under indications. The transfusion autoerythrocytes was made according to reacting at the moment of carrying out of work to orders of Ministry of Health of the Russian Federation: to the Order of Ministry of Health of the Russian Federation from November, 25th, 2002 No 363 "About the statement of the Instruction on application of components of blood" and to the Order of Ministry of Health of the Russian Federation from April, 2nd, 2013 N 183n "About the statement of rules of clinical use of donor blood and (or) its components".

RESULTS

Total 122 newborns received an autotransfiusion of washed erythrocytes of placental/umbilical cord blood for the correction of anemia in the department of neonatal surgery in the period from 2005 to 2013. 66 children who are in the first two weeks of life were performed surgical intervention for malformation of the gastrointestinal tract (gastorshizis (22), omphalocele (2). itestinal atresia (10), esophageal atresia or doubling (4)), congenital diaphragmatic hernia (15), space-occupying lesions (teratoma (6) and lymphangioma (3)) and other pathologies (adenomatous lung (1), the sequestration of the lung (2). ovarian cyst (1)). Control group consisted of39 infants operated on for similar malformations , which in the absence of prior communication harvested autologous red blood cells in the first three weeks of life sparkled donor erythrocytes. Inmost cases (57 newborns - 86.4%) of the amount harvested and transfused blood autokonmpo tov was sufficient for the relief ofanemia, despite the fact that the volume of transfused autoeritrotsitnoy mass per kilogram of body weight was almost two times lower than the amount of donor erythrocyte mass used in the comparison group. Additional donor transfusion of red blood cells in the group of children who had autotransfusion, it took nine newborns (13.6%). The main indications for repeated transfusions were clinical and laboratory signs of anemia, persisting after autotransfusion or resulting from repeated operations. After transfusion of washed autoerythrocytes value of clinical and biochemical. blood tests, urinalysis were within the age norm. Post-transfusion reactions in children who have received a transfusion autoerythrocytes not mentioned

CONCLUSION

The use of placental/umbilical cord blood autoe,ythrocyvtes in children requiring early surgical correction of congenital malformations can significantly reduce the need for donor red blood cells. Autologous red blood cells use is a safe and effective alternative to transfusion of donor red blood cells.