Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5832-5840. doi: 10.1099/ijsem.0.004481.
As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species. The isolates were Gram-staining-positive, coccoid, facultatively anaerobic, non-motile, catalase-negative and oxidase-negative. Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus , with and as closest relatives. Results indicated that they represented two different novel species with two strains, (TMW 2.1612/TMW 2.1613 and TMW 2.1615/TMW 2.1614), respectively. The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation. The 16S rRNA gene sequences of TMW 2.1612 and TMW 2.1615 had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of , respectively. However, the ANIb value between the respective type strains TMW 2.1612 and TMW 2.1615, and the type strain of was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612 and TMW 2.1615. The DDH estimate value between the respective type strain TMW 2.1612 and TMW 2.1615 and the most closely related type strain of was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612 and TMW 2.1615. Peptidoglycan type of strain TMW 2.1612 is Lys-Thr-Ala and major fatty acids are summed feature 8 and C. Peptidoglycan type of strain TMW 2.1615 is Lys-Ala and major fatty acids are C, Ccyclo ω8 and summed feature 8. On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus , for which the names sp. nov and sp. nov are proposed. The designated respective type strains are TMW 2.1612 (=DSM 111016 =CECT 30115) and TWM 2.1615 (=DSM 111017 =CECT 30116).
作为研究德国改良气氛包装牛肉腐败微生物组的一部分，分离出了四种新型乳酸菌，并通过多相方法对其进行了分类学特征描述，结果表明它们不能被分配到已知的物种中。这些分离株革兰氏阳性、球菌、兼性厌氧、非运动、过氧化氢酶阴性和氧化酶阴性。形态学、生理学和系统发育分析显示，在属内存在一个独特的谱系，与 和 最为密切相关。结果表明，它们代表了两个不同的新种，其中两个菌株（TMW 2.1612/TMW 2.1613 和 TMW 2.1615/TMW 2.1614）分别代表两个不同的新种。这两个新种的两个菌株具有相同的 16S rRNA 基因序列，但 MLSA 允许其种内分化。TMW 2.1612 和 TMW 2.1615 的 16S rRNA 基因序列彼此相似性为 99.85%，与最密切相关的 型菌株的相似性分别为 99.85%和 99.78%。然而，相应的 TMW 2.1612 和 TMW 2.1615 型菌株与 型菌株之间的 ANIb 值分别仅为 94.3%和 92.0%，而 TMW 2.1612 和 TMW 2.1615 之间的 ANIb 值为 92.9%。相应的 TMW 2.1612 和 TMW 2.1615 型菌株与最密切相关的 型菌株的 DDH 估计值分别仅为 59.9%和 48.9%，而 TMW 2.1612 和 TMW 2.1615 之间的 DDH 估计值为 51.1%。TMW 2.1612 菌株的肽聚糖类型为 Lys-Thr-Ala，主要脂肪酸为特征 8 和 C。TMW 2.1615 菌株的肽聚糖类型为 Lys-Ala，主要脂肪酸为 C、Ccyclo ω8 和特征 8。基于多相证据，这些肉类分离株代表了属的两个新种，分别提议命名为 sp. nov 和 sp. nov。指定的相应模式菌株分别为 TMW 2.1612（=DSM 111016 =CECT 30115）和 TWM 2.1615（=DSM 111017 =CECT 30116）。
