Siegień Irena, Adamczuk Aneta, Wróblewska Katarzyna
Institute of Biology, The University of Bialystok, Świerkowa 20b, 15-950 Bialystok, Poland.
Acta Physiol Plant. 2013;35(3):781-789. doi: 10.1007/s11738-012-1118-4. Epub 2012 Oct 12.
The relationships between organogenesis of oil flax ( L., cv. 'Szafir') in vitro, cyanogenic potential (HCN-p) of these tissues and light were investigated. Shoot multiplication obtained on Murashige and Skoog medium containing 0.05 mg L 2,4-dichloro-phenoxyacetic acid and 1 mg L 6-benzyladenine (BA), was about twice higher in light-grown cultures than those in darkness. Light-grown explants showed also higher rate of roots regeneration (in medium containing 1 mg L α-naphtaleneacetic acid and 0.05 mg L BA) than dark-grown ones. The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific and generally was higher under light conditions than in darkness. The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots, and its amount was twice as high as in roots, and about threefold higher than in callus tissue. The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness. Thus, higher frequency of regeneration of light-grown cultures than dark-grown ones seems to be correlated with higher HCN-p of these tissues. We suggest that free HCN, released from cyanoglucosides potentially at higher level under light conditions, may be involved in some organogenetic processes which improve regeneration efficiency.
研究了油用亚麻（L.，品种‘Szafir’）离体器官发生、这些组织的生氰潜力（HCN-p）与光照之间的关系。在含有0.05 mg/L 2,4-二氯苯氧乙酸和1 mg/L 6-苄基腺嘌呤（BA）的Murashige和Skoog培养基上获得的芽增殖，在光照培养的外植体中比黑暗培养的约高两倍。光照培养的外植体（在含有1 mg/L α-萘乙酸和0.05 mg/L BA的培养基中）也比黑暗培养的外植体表现出更高的生根率。离体培养的亚麻的生氰潜力（以亚麻苦苷和百脉根苷含量以及亚麻苦苷酶活性表示）具有组织特异性，并且一般在光照条件下比黑暗中更高。在光照再生的芽中检测到最高浓度的亚麻苦苷和百脉根苷，其含量是根中的两倍，比愈伤组织中的高约三倍。光照再生器官中亚麻苦苷酶和β-氰基丙氨酸合酶的活性也高于黑暗中的。因此，光照培养的外植体比黑暗培养的具有更高的再生频率，这似乎与这些组织更高的HCN-p相关。我们认为，在光照条件下可能从氰苷中释放出的游离HCN，可能参与了一些提高再生效率的器官发生过程。
