Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland.
Medical and Molecular Microbiology Unit "Emerging Antibiotic Resistance", Dept of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
Antimicrob Resist Infect Control. 2015 Mar 26;4:9. doi: 10.1186/s13756-015-0051-x. eCollection 2015.
The emergence and worldwide spread of carbapenemase-producing Enterobacteriaceae is of great concern to public health services. The aim of this study was to investigate the occurrence of carbapenemase-producing Enterobacteriaceae in the community in Switzerland.
One thousand and eighty-six stool samples of healthy humans (staff members of a food-processing company which were screened for the occurrence of salmonellae) were collected in September 2014. After an initial enrichment-step, carbapenemase-producing Enterobacteriaceae were isolated from the carbapenem-containing selective medium SUPERCARBA II. Grown colonies from 11 samples were screened by PCR for the presence of bla KPC, bla NDM, bla OXA-48 and bla VIM. A single OXA-48-producing Escherichia coli was detected. Phylogenetic grouping and multi-locus sequence typing (MLST) revealed that this strain belonged to D:ST38, a type which had been previously reported in the UK, France, Lebanon and Egypt.
The results of this study show that OXA-48-producing Enterobacteriaceae have started to spread into the community in Switzerland, and a continuous monitoring is necessary to better understand their dissemination in the human population as well as in animals and the environment.
能够产生碳青霉烯酶的肠杆菌科的出现和在世界范围内的传播引起了公共卫生服务部门的极大关注。本研究的目的是调查瑞士社区中产碳青霉烯酶的肠杆菌科的发生情况。
2014 年 9 月，采集了 1086 份健康人类的粪便样本（食品加工公司的员工，他们接受了沙门氏菌发生情况的筛查）。在初始富集步骤后，从含有碳青霉烯的选择性培养基 SUPERCARBA II 中分离出产碳青霉烯酶的肠杆菌科。从 11 个样本中生长的菌落通过 PCR 筛选 bla KPC、bla NDM、bla OXA-48 和 bla VIM 的存在。检测到一株单独的产 OXA-48 的大肠杆菌。系统发生分组和多位点序列分型（MLST）显示，该菌株属于 D:ST38，该类型先前在英国、法国、黎巴嫩和埃及有报道过。
本研究结果表明，产 OXA-48 的肠杆菌科已开始在瑞士社区传播，需要进行持续监测，以更好地了解它们在人群、动物和环境中的传播情况。
