Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109 USA.
Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 USA.
Microbiome. 2015 Apr 1;3:12. doi: 10.1186/s40168-015-0074-9. eCollection 2015.
Recent work indicates that the airways of persons with cystic fibrosis (CF) typically harbor complex bacterial communities. However, the day-to-day stability of these communities is unknown. Further, airway community dynamics during the days corresponding to the onset of symptoms of respiratory exacerbation have not been studied.
Using 16S rRNA amplicon sequencing of 95 daily sputum specimens collected from four adults with CF, we observed varying degrees of day-to-day stability in airway bacterial community structures during periods of clinical stability. Differences were observed between study subjects with respect to the degree of community changes at the onset of exacerbation. Decreases in the relative abundance of dominant taxa were observed in three subjects at exacerbation. We observed no relationship between total bacterial load and clinical status and detected no viruses by multiplex PCR.
CF airway microbial communities are relatively stable during periods of clinical stability. Changes in microbial community structure are associated with some, but not all, pulmonary exacerbations, supporting previous observations suggesting that distinct types of exacerbations occur in CF. Decreased abundance of species that are dominant at baseline suggests a role for less abundant taxa in some exacerbations. Daily sampling revealed patterns of change in microbial community structures that may prove useful in the prediction and management of CF pulmonary exacerbations.
最近的研究表明，囊性纤维化（CF）患者的气道通常存在复杂的细菌群落。然而，这些群落的日常稳定性尚不清楚。此外，在呼吸道感染加重症状发作期间的对应日子里，气道群落动态变化尚未被研究。
通过对 4 名 CF 成年患者的 95 份日常痰标本进行 16S rRNA 扩增子测序，我们观察到在临床稳定期，气道细菌群落结构存在不同程度的日常稳定性。在加重期开始时，研究对象之间的群落变化程度存在差异。在 3 名患者的加重期，观察到优势菌群相对丰度下降。我们未发现总细菌负荷与临床状态之间存在关系，也未通过多重 PCR 检测到病毒。
CF 气道微生物群落在临床稳定期相对稳定。微生物群落结构的变化与部分但不是所有的肺部加重有关，这支持了先前的观察结果，即 CF 中发生不同类型的加重。在基线时占主导地位的物种丰度下降表明，在某些加重中，较少丰度的类群可能起作用。每日采样揭示了微生物群落结构变化的模式，这可能有助于 CF 肺部加重的预测和管理。
