通过电穿孔法对苏云金芽孢杆菌营养细胞进行转化。

Transformation of Bacillus thuringiensis vegetative cells by electroporation.

作者信息

Masson L, Préfontaine G, Brousseau R

机构信息

National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.

出版信息

FEMS Microbiol Lett. 1989 Aug;51(3):273-7. doi: 10.1016/0378-1097(89)90409-6.

DOI:10.1016/0378-1097(89)90409-6

PMID:2583476

Abstract

A highly efficient procedure for the transformation of Bacillus thuringiensis and Bacillus subtilis using covalently closed circular plasmid DNA was developed by using the small Staphylococcus aureus plasmid pC194 and electroporation. We have achieved transformation efficiencies in B. thuringiensis subsp. kurstaki (HD-73) greater than 5 x 10(6) transformants/micrograms plasmid DNA. The electro-transformation (or electroporation) procedure also worked with B. subtilis 168 although at a 200-fold less level of efficiency. The results indicated that the plasmid exists in double and single-stranded forms both in B. subtilis and B. thuringiensis. A second single-stranded species was also observed in both species. This technique may prove to be applicable to other members of the genus Bacillus.

摘要

利用小的金黄色葡萄球菌质粒pC194和电穿孔技术，开发了一种使用共价闭合环状质粒DNA转化苏云金芽孢杆菌和枯草芽孢杆菌的高效方法。我们在苏云金芽孢杆菌库斯塔克亚种（HD-73）中实现了大于5×10⁶转化子/微克质粒DNA的转化效率。电转化（或电穿孔）方法对枯草芽孢杆菌168也有效，尽管效率低200倍。结果表明，该质粒在枯草芽孢杆菌和苏云金芽孢杆菌中均以双链和单链形式存在。在这两个物种中还观察到了第二种单链物种。该技术可能被证明适用于芽孢杆菌属的其他成员。