State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China.
Int J Mol Sci. 2021 Dec 31;23(1):444. doi: 10.3390/ijms23010444.
RNA interference (RNAi) has been developed and used as an emerging strategy for pest management. Here, an entomopathogen () was used to express the dsRNA for the control of . A vector containing a 325-bp fragment of the conserved region of arginine kinase gene () flanking in two ends with the promoter Pro3α was developed and transferred into 8010 and BMB171, and consequently engineered strains 8010AKi and BMB171AKi expressing dsRNA of were developed. The two engineered strains were separately mixed with 8010 in a series of ratios, and then fed to the larvae. We found that 8010:8010AKi of 9:1 and 8010:BMB171AKi of 7:3 caused a higher mortality than 8010. expression levels in the individuals treated with the mixtures, 8010AKi and BMB171Aki, were lower than that in the control. The intrinsic rate of increase () and net reproductive rate () of the population treated with 8010:8010AKi of 9:1 were lower than those of the population treated with 8010 or 8010AKi. We developed a -mediated insect RNAi for the control of and demonstrated a practical approach to integrating the entomopathogen with RNAi technique for the pest management.
RNA 干扰 (RNAi) 已被开发并用作害虫管理的新兴策略。在这里，利用一种昆虫病原体 () 来表达 dsRNA，以控制 。构建了一个含有 325bp 保守区片段的载体，侧翼两端带有启动子 Pro3α，该片段来自 精氨酸激酶基因 ()。将该载体转入 8010 和 BMB171，从而构建了表达 的 dsRNA 的工程菌株 8010AKi 和 BMB171AKi。将这两种工程菌株分别与 8010 以一系列比例混合，然后喂食 幼虫。我们发现，8010:8010AKi 的 9:1 和 8010:BMB171AKi 的 7:3 比 8010 引起更高的死亡率。混合物处理个体中的 表达水平，8010AKi 和 BMB171Aki，低于对照。处理 8010:8010AKi 的 9:1 的种群的内禀增长率 () 和净生殖率 () 低于处理 8010 或 8010AKi 的种群。我们开发了一种基于 的昆虫 RNAi 来控制 ，并证明了将昆虫病原体与 RNAi 技术相结合用于害虫管理的实用方法。
