弗氏枸橼酸杆菌O24:H8（菌株CNCTC 92/89）产生的脂多糖中O-特异性多糖和核心寡糖的核磁共振研究。

Lundqvist Lena C E, Kaszowska Marta, Sandström Corine

Department of Chemistry and Biotechnology, Uppsala BioCenter Swedish University of Agricultural Sciences, P.O. Box 7015, Uppsala SE-750-07, Sweden.

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, Wroclaw PL-53-114, Poland.

Molecules. 2015 Apr 1;20(4):5729-39. doi: 10.3390/molecules20045729.

The structures of the O-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from Plesiomonas shigelloides O24:H8, strain CNCTC 92/89, have been investigated by NMR spectroscopy and ESI mass spectrometry. The O-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [→3)-α-FucpNAc-(1→3)-α-GalpNAcA-(1→3)-α-QuipNAc-(1→] and of α-RhapNAc (1→4) linked to the GalpNAcA residue. An identical structure has been reported for the capsular polysaccharide of the clinical isolate of Vibrio vulnificus strain BO62316 [1]. The core oligosaccharide was composed of a decasaccharide which structure is identical with these in P. shigelloides serotype O54 [2] and serotype O37 [3].

通过核磁共振光谱法和电喷雾电离质谱法对类志贺邻单胞菌O24:H8菌株CNCTC 92/89脂多糖的O-特异性多糖和核心寡糖结构进行了研究。发现O-特异性多糖由一个四糖重复单元组成，该重复单元由[→3)-α-岩藻糖胺-(1→3)-α-氨基半乳糖醛酸-(1→3)-α-喹诺糖胺-(1→]和与氨基半乳糖醛酸残基相连的α-鼠李糖胺(1→4)组成。创伤弧菌菌株BO62316临床分离株的荚膜多糖也报道了相同的结构[1]。核心寡糖由一个十糖组成，其结构与类志贺邻单胞菌O54血清型[2]和O37血清型[3]中的结构相同。