Maciejewska Anna, Lukasiewicz Jolanta, Niedziela Tomasz, Szewczuk Zbigniew, Lugowski Czeslaw
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland.
Carbohydr Res. 2009 May 12;344(7):894-900. doi: 10.1016/j.carres.2009.02.020. Epub 2009 Feb 28.
Plesiomonasshigelloides strain CNCTC 110/92 (O51) was identified as a new example of plesiomonads synthesising lipopolysaccharides (LPSs) that show preference for a non-aqueous surrounding during phenol/water extraction. Chemical analyses combined with (1)H and (13)C NMR spectroscopy, MALDI-TOF and ESI mass spectrometry showed that the repeating units of the O-specific polysaccharides isolated from phenol and water phase LPSs of P. shigelloides O51 have the same structure: -->4)-beta-D-GlcpNAc3NRA-(1-->4)-alpha-L-FucpAm3OAc-(1-->3)-alpha-D-QuipNAc-(1-->, containing the rare sugar constituent 2,3-diamino-2,3-dideoxyglucuronic acid (GlcpNAc3NRA), and substituents such as D-3-hydroxybutyric acid (R) and acetamidino group (Am). The HR-MAS NMR spectra obtained for the isolated LPSs and directly on bacteria indicated that the O-acetylation pattern was consistent throughout the entire preparation. The (1)H chemical shift values of the structure reporter groups identified in the isolated O-antigens matched those present in bacteria. We have found that the O-antigens recovered from the phenol phase showed a higher degree of polymerisation than those isolated from the water phase.
类志贺邻单胞菌菌株CNCTC 110/92（O51）被鉴定为合成脂多糖（LPS）的邻单胞菌新实例，该脂多糖在酚/水提取过程中表现出对非水环境的偏好。化学分析结合¹H和¹³C NMR光谱、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和电喷雾电离质谱（ESI）表明，从类志贺邻单胞菌O51的酚相和水相LPS中分离出的O-特异性多糖的重复单元具有相同结构：→4)-β-D-2,3-二氨基-2,3-二脱氧葡萄糖醛酸-(1→4)-α-L-3-O-乙酰基岩藻糖胺-(1→3)-α-D-2,3-二氨基-2,3-二脱氧喹诺糖-(1→，含有稀有糖成分2,3-二氨基-2,3-二脱氧葡萄糖醛酸（GlcpNAc3NRA）以及D-3-羟基丁酸（R）和脒基（Am）等取代基。对分离出的LPS以及直接在细菌上获得的高分辨魔角旋转NMR光谱表明，整个制剂中的O-乙酰化模式是一致的。在分离出的O-抗原中鉴定出的结构报告基团的¹H化学位移值与细菌中存在的化学位移值相匹配。我们发现，从酚相中回收的O-抗原比从水相中分离出的O-抗原具有更高的聚合度。
