Siems W, Kowalewski J, Werner A, Schimke I, Gerber G
Institut für Biochemie, Bereich Medizin (Charité), Humboldt-Universität zu Berlin, DDR.
Free Radic Res Commun. 1989;7(3-6):347-53. doi: 10.3109/10715768909087961.
Oxidative loading during the reperfusion of the proximal jejunum of rats following a one hour-period of complete ischemia was demonstrated in in vivo-experiments by the increases of the GSSG: total glutathione ratio and the concentration of TBA-RS. The pretreatment of the animals with the xanthine oxidoreductase inhibitor allopurinol diminished the accumulation of GSSG and of TBA-RS. It was concluded that the purine nucleotide degradation is an important source of oxygen reduction products in reoxygenated small intestine. The tissue concentrations of nucleotides, nucleosides and nucleobases were measured by an ion-pair reversed-phase HPLC separation. There occurred fast declines of ATP and GTP concentrations during ischaemia leading to temporary increases of nucleoside mono- and diphosphate pools. The hypoxanthine concentration is increased about twentyfold during oxygen deficiency. The ATP and GTP restoration during the reperfusion was accelerated in presence of allopurinol. The shares of the beneficial allopurinol effects are not yet clarified.
在大鼠空肠近端完全缺血1小时后的再灌注过程中，体内实验通过谷胱甘肽二硫化物与总谷胱甘肽比值的增加以及硫代巴比妥酸反应物（TBA-RS）浓度的升高，证实了氧化负荷的存在。用黄嘌呤氧化还原酶抑制剂别嘌呤醇对动物进行预处理，可减少谷胱甘肽二硫化物和TBA-RS的积累。得出的结论是，嘌呤核苷酸降解是复氧小肠中氧还原产物的重要来源。通过离子对反相高效液相色谱分离法测量了核苷酸、核苷和核碱基的组织浓度。缺血期间ATP和GTP浓度迅速下降，导致核苷单磷酸和二磷酸池暂时增加。缺氧期间次黄嘌呤浓度增加约20倍。在别嘌呤醇存在的情况下，再灌注期间ATP和GTP的恢复加速。别嘌呤醇有益作用的份额尚未阐明。
