Tachon P
Laboratoires de Recherche Fondamentale de l'Oreal, Aulnay-Sous-Bois, France.
Free Radic Res Commun. 1989;7(3-6):367-74. doi: 10.3109/10715768909087963.
The hypoxanthine - xanthine oxidase system generates an extracellular flux of superoxide anion radical (O2.-) and hydrogen peroxide (H2O2). Catalase but not superoxide dismutase (SOD) protects V79 cells exposed to the hypoxanthine - xanthine oxidase system, showing that H2O2 is the major reactive oxygen species involved in the cytotoxicity of such a system. In contrast to SOD, the lipophilic SOD like compound CuII (diisopropylsalicylate)2 (CuDIPS) exhibits some protection at non cytotoxic concentration. It is also found that methanol partially protects cells exposed to the hypoxanthine-xanthine oxidase system. It appears that in our experimental conditions (temperature, ionic strength and pH) the protective effect afforded by methanol and CuDIPS is due to the inhibition of the xanthine oxidase activity.
次黄嘌呤-黄嘌呤氧化酶系统会产生细胞外超氧阴离子自由基（O2.-）和过氧化氢（H2O2）通量。过氧化氢酶而非超氧化物歧化酶（SOD）可保护暴露于次黄嘌呤-黄嘌呤氧化酶系统的V79细胞，这表明H2O2是该系统细胞毒性中主要的活性氧物质。与SOD不同，亲脂性类SOD化合物CuII（二异丙基水杨酸盐）2（CuDIPS）在非细胞毒性浓度下具有一定保护作用。还发现甲醇可部分保护暴露于次黄嘌呤-黄嘌呤氧化酶系统的细胞。在我们的实验条件（温度、离子强度和pH）下，甲醇和CuDIPS的保护作用似乎是由于抑制了黄嘌呤氧化酶的活性。
