Emerit I, Khan S H, Cerutti P A
J Free Radic Biol Med. 1985;1(1):51-7. doi: 10.1016/0748-5514(85)90029-7.
Culture medium of lymphocyte cultures that have been exposed to the superoxide generating system hypoxanthine plus xanthine oxidase (X-XO) contains substances with chromosome damaging properties. This is demonstrated by the ability of ultrafiltrates of such culture media to induce chromosomal aberrations and sister chromatid exchanges in the lymphocytes of blood test cultures. Culture medium becomes active about 15 hours after the addition of X-XO and stimulation by phytohemagglutinin. Concomitant with the accumulation of clastogenic material, assays for conjugated dienes and thiobarbituric acid-reactive material which measure lipid-peroxidation become positive in the culture media. When cells are pretreated with superoxide dismutase or glutathione peroxidase before the addition of X-XO neither clastogenic substances nor lipid peroxidation products are detected. Catalase is a less efficient protector.
暴露于超氧化物生成系统次黄嘌呤加黄嘌呤氧化酶（X-XO）的淋巴细胞培养物的培养基中含有具有染色体损伤特性的物质。这种物质可通过此类培养基的超滤物在血液检测培养的淋巴细胞中诱导染色体畸变和姐妹染色单体交换的能力得到证明。在添加X-XO并经植物血凝素刺激后约15小时，培养基开始变得活跃。伴随着致裂物质的积累，用于测量脂质过氧化的共轭二烯和硫代巴比妥酸反应性物质的检测在培养基中呈阳性。当在添加X-XO之前用超氧化物歧化酶或谷胱甘肽过氧化物酶对细胞进行预处理时，既检测不到致裂物质，也检测不到脂质过氧化产物。过氧化氢酶是一种效果较差的保护剂。
