Scholten D, Trebicka J, Liedtke C, Weiskirchen R
Department of Internal Medicine III, RWTH University Hospital Aachen, Aachen, Germany.
Department of Internal Medicine I, University Hospital Bonn, Bonn, Germany.
Lab Anim. 2015 Apr;49(1 Suppl):4-11. doi: 10.1177/0023677215571192.
Recently, the need for more standardized operation procedures in experimental liver fibrosis research was suggested due to dramatic changes in European animal welfare rules. Here, we present a short series of standard operation procedures (SOPs) summarizing the most relevant and widely accepted experimental models for the induction of liver injury leading to liver fibrosis. The described procedures are based on the long-term experience of the Collaborative Research Centre 'Organ Fibrosis: From Mechanisms of Injury to Modulation of Disease' (http://www.sfbtrr57.rwth-aachen.de/), which is supported by the German Research Foundation (SFB/TRR57). These SOPs will help to improve standardization of fibrosis models and to increase the comparability of data between different laboratories with the aim of reducing animal experimentation according to the principle that was proposed in 1959 by Russell and Burch as an ethical framework for conducting scientific experiments with animals, namely the replacement, refinement and reduction (3R) principle. In the first section we focus on the carbon tetrachloride (CCl4) model in mice, which is the toxic model of liver fibrosis induction most commonly used worldwide.
最近，由于欧洲动物福利规则发生了巨大变化，有人提出在实验性肝纤维化研究中需要更标准化的操作程序。在此，我们介绍一系列简短的标准操作程序（SOP），总结了用于诱导导致肝纤维化的肝损伤的最相关且广泛接受的实验模型。所描述的程序基于“器官纤维化：从损伤机制到疾病调控”合作研究中心（http://www.sfbtrr57.rwth-aachen.de/）的长期经验，该中心由德国研究基金会（SFB/TRR57）资助。这些SOP将有助于提高纤维化模型的标准化程度，并增加不同实验室之间数据的可比性，目的是根据1959年罗素和伯奇提出的作为动物科学实验伦理框架的原则，即替代、优化和减少（3R）原则，减少动物实验。在第一部分中，我们重点介绍小鼠四氯化碳（CCl4）模型，这是全球最常用的肝纤维化诱导毒性模型。
