Willrodt Christian, Karande Rohan, Schmid Andreas, Julsing Mattijs K
Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany.
Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany.
Curr Opin Biotechnol. 2015 Dec;35:52-62. doi: 10.1016/j.copbio.2015.03.010. Epub 2015 Apr 1.
The recent progress in sustainable chemistry and in synthetic biology increased the interest of chemical and pharmaceutical industries to implement microbial processes for chemical synthesis. However, most organisms used in biotechnological applications are not evolved by Nature for the production of hydrophobic, non-charged, volatile, or toxic compounds. In order to overcome this discrepancy, bioprocess design should consist of an integrated approach addressing pathway, cellular, reaction, and process engineering. Highlighting selected examples, we show that surprisingly often Nature provides conceptual solutions to enable chemical synthesis. Complemented by established methods from (bio)chemical and metabolic engineering, these concepts offer potential strategies yet to be explored and translated into innovative technical solutions enabling sustainable microbial production of non-natural chemicals.
可持续化学和合成生物学的最新进展激发了化学和制药行业对采用微生物过程进行化学合成的兴趣。然而，生物技术应用中使用的大多数生物体并非自然进化而来用于生产疏水性、不带电荷、挥发性或有毒化合物的。为了克服这一差异，生物过程设计应采用一种综合方法，涵盖途径、细胞、反应和过程工程。通过突出选定的例子，我们表明，自然界常常出人意料地提供了实现化学合成的概念性解决方案。这些概念辅以（生物）化学和代谢工程的既定方法，提供了有待探索的潜在策略，并可转化为创新的技术解决方案，以实现非天然化学品的可持续微生物生产。
