Hemmasizadeh Ali, Tsamis Alkiviadis, Cheheltani Rabee, Assari Soroush, D'Amore Antonio, Autieri Michael, Kiani Mohammad F, Pleshko Nancy, Wagner William R, Watkins Simon C, Vorp David, Darvish Kurosh
Departments of Mechanical Engineering, Temple University, Philadelphia, USA.
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
J Mech Behav Biomed Mater. 2015 Jul;47:12-20. doi: 10.1016/j.jmbbm.2015.03.004. Epub 2015 Mar 19.
Determination of correlations between transmural mechanical and morphological properties of aorta would provide a quantitative baseline for assessment of preventive and therapeutic strategies for aortic injuries and diseases. A multimodal and multidisciplinary approach was adopted to characterize the transmural morphological properties of descending porcine aorta. Histology and multi-photon microscopy were used for describing the media layer micro-architecture in the circumferential-radial plane, and Fourier Transform infrared imaging spectroscopy was utilized for determining structural protein, and total protein content. The distributions of these quantified properties across the media thickness were characterized and their relationship with the mechanical properties from a previous study was determined. Our findings indicate that there is an increasing trend in the instantaneous Young׳s modulus (E), elastic lamella density (ELD), structural protein (SPR), total protein (TPR), and elastin and collagen circumferential percentage (ECP and CCP) from the inner towards the outer layers. Two regions with equal thickness (inner and outer halves) were determined with significantly different morphological and material properties. The results of this study represent a substantial step toward anatomical characterization of the aortic wall building blocks and establishment of a foundation for quantifying the role of microstructural components on the functionality of aorta.
确定主动脉跨壁力学特性与形态学特性之间的相关性，将为评估主动脉损伤和疾病的预防及治疗策略提供定量基线。采用多模态和多学科方法来表征猪降主动脉的跨壁形态学特性。组织学和多光子显微镜用于描述中膜层在周向-径向平面的微观结构，傅里叶变换红外成像光谱用于测定结构蛋白和总蛋白含量。表征了这些量化特性在整个中膜厚度上的分布，并确定了它们与先前研究中力学特性的关系。我们的研究结果表明，从内层到外层，瞬时杨氏模量（E）、弹性板层密度（ELD）、结构蛋白（SPR）、总蛋白（TPR）以及弹性蛋白和胶原蛋白周向百分比（ECP和CCP）呈上升趋势。确定了两个厚度相等的区域（内半层和外半层），其形态学和材料特性存在显著差异。本研究结果朝着主动脉壁构建块的解剖学表征迈出了重要一步，并为量化微观结构成分对主动脉功能的作用奠定了基础。
