Brooks Eric R, Wallingford John B
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States.
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States; The Howard Hughes Medical Institute, Chevy Chase, MD, USA.
Methods Cell Biol. 2015;127:131-59. doi: 10.1016/bs.mcb.2015.01.018. Epub 2015 Mar 9.
Cilia are key organelles in development and homeostasis. The ever-expanding complement of cilia associated proteins necessitates rapid and tractable models for in vivo functional investigation. Xenopus laevis provides an attractive model for such studies, having multiple ciliated populations, including primary and multiciliated tissues. The rapid external development of Xenopus and the large cells make it an especially excellent platform for imaging studies. Here we present embryological and cell biological methods for the investigation of cilia structure and function in X. laevis, with a focus on quantitative live and fixed imaging.
纤毛是发育和体内平衡中的关键细胞器。与纤毛相关的蛋白质不断增加，这就需要快速且易于处理的模型来进行体内功能研究。非洲爪蟾为这类研究提供了一个有吸引力的模型，它有多种纤毛群体，包括初级纤毛组织和多纤毛组织。非洲爪蟾快速的体外发育过程以及其较大的细胞使其成为成像研究的一个特别出色的平台。在这里，我们介绍用于研究非洲爪蟾纤毛结构和功能的胚胎学和细胞生物学方法，重点是定量实时成像和固定样本成像。
