He Linling, Zhu Jiang
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA.
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Curr Opin Virol. 2015 Apr;11:103-12. doi: 10.1016/j.coviro.2015.03.013. Epub 2015 Mar 31.
Rational approaches will be required to develop universal vaccines for viral pathogens such as human immunodeficiency virus, hepatitis C virus, and influenza, for which empirical approaches have failed. The main objective of a rational vaccine strategy is to design novel immunogens that are capable of inducing long-term protective immunity. In practice, this requires structure-based engineering of the target neutralizing epitopes and a quantitative readout of vaccine-induced immune responses. Therefore, computational tools that can facilitate these two areas have played increasingly important roles in rational vaccine design in recent years. Here we review the computational techniques developed for protein structure prediction and antibody repertoire analysis, and demonstrate how they can be applied to the design and evaluation of epitope vaccines.
对于人类免疫缺陷病毒、丙型肝炎病毒和流感等病毒病原体，经验性方法已告失败，因此需要合理的方法来开发通用疫苗。合理疫苗策略的主要目标是设计能够诱导长期保护性免疫的新型免疫原。实际上，这需要基于结构对目标中和表位进行工程设计，并对疫苗诱导的免疫反应进行定量测定。因此，近年来能够推动这两个领域发展的计算工具在合理疫苗设计中发挥了越来越重要的作用。在此，我们综述了为蛋白质结构预测和抗体库分析而开发的计算技术，并展示了它们如何应用于表位疫苗的设计和评估。
