Steyger P S, Furness D N, Hackney C M, Richardson G P
Department of Communication and Neuroscience, University of Keele, Staffordshire, U.K.
Hear Res. 1989 Oct;42(1):1-16. doi: 10.1016/0378-5955(89)90113-5.
The distribution of tubulin has been investigated in surface preparations of the guinea pig organ of Corti using indirect immunofluorescence microscopy. Two different monoclonal antibodies to tubulin produce similar distinct patterns of labelling in hair cells. Labelling is greater in inner hair cells than outer hair cells. It occurs in rings around the cell apex, and in a meshwork below and channels through, the cuticular plate. In outer hair cells from the apical region of the cochlea, labelling occurs around the location of a basalward protrusion of the cuticular plate. These patterns correlate with the location of microtubules observed using transmission electron microscopy. A large patch of labelling occurs on the strial side of the cell corresponding to the largest channel through the cuticular plate and the kinociliary basal body. Strands of labelling are seen running parallel to the long axis of the cell between the subcuticular and synaptic region. Many more of these strands are seen in the inner hair cell than the outer hair cell and may correspond to tracks of microtubules transporting neurotransmitter vesicles or other organelles. In outer hair cells, intense labelling and many microtubules are seen in the subnuclear region. The possible roles of the different microtubule arrangements are discussed.
利用间接免疫荧光显微镜技术，对豚鼠柯蒂氏器的表面制剂中的微管蛋白分布进行了研究。两种不同的抗微管蛋白单克隆抗体在毛细胞中产生相似的独特标记模式。内毛细胞中的标记比外毛细胞中的更强。标记出现在细胞顶端周围的环中，以及角质板下方的网状结构和穿过角质板的通道中。在耳蜗顶端区域的外毛细胞中，标记出现在角质板向基底突出部位的周围。这些模式与使用透射电子显微镜观察到的微管位置相关。在细胞的血管纹侧出现一大片标记，对应于穿过角质板和动纤毛基体的最大通道。在皮下和突触区域之间，可见标记条带平行于细胞的长轴延伸。在内毛细胞中比在外毛细胞中看到更多这样的条带，它们可能对应于运输神经递质囊泡或其他细胞器的微管轨迹。在外毛细胞中，在核下区域可见强烈的标记和许多微管。讨论了不同微管排列的可能作用。
