Saint Marie R L, Morest D K, Brandon C J
Department of Anatomy, University of Connecticut Health Center, Farmington 06032.
Hear Res. 1989 Oct;42(1):97-112. doi: 10.1016/0378-5955(89)90120-2.
The distribution of GABAergic endings was examined histochemically in the ventral cochlear nucleus (VCN) of the cat using an antibody to glutamate decarboxylase (GAD), the synthetic enzyme for GABA. Immunoreactive (GAD+) endings appeared in all subdivisions of the cat VCN. Each of the principal cell types had a characteristic labeling pattern, based on the size, concentration, and distribution of GAD+ endings on its soma. Spherical bushy cell somata were typically contacted by many small (less than 1.5 microns in diameter) and medium-sized (1.5-2 microns in diameter) endings, many of which aggregated into tight clusters. Globular bushy cells had a similar pattern, but the clusters of GAD+ endings were less tightly packed. Reactive endings on stellate cells were more evenly distributed. GAD+ endings on octopus cells were larger (up to 2.5 microns in diameter) than those on the bushy cells and tended to aggregate into small clusters or rows on the somata and dendrites. Reactive endings contained small pleomorphic vesicles and formed symmetrical synaptic contacts on each of the cell types examined. The patterns formed by GAD+ endings on each type of neuron resemble those of certain types of non-cochlear axons previously described with the Golgi methods as projecting from the dorsal cochlear nucleus and the trapezoid body.
使用针对γ-氨基丁酸（GABA）合成酶谷氨酸脱羧酶（GAD）的抗体，通过组织化学方法研究了猫腹侧耳蜗核（VCN）中GABA能末梢的分布。免疫反应性（GAD+）末梢出现在猫VCN的所有亚区。根据GAD+末梢在其胞体上的大小、浓度和分布，每种主要细胞类型都有其特征性的标记模式。球形毛细胞的胞体通常被许多小（直径小于1.5微米）和中等大小（直径1.5 - 2微米）的末梢所接触，其中许多聚集形成紧密的簇。球状毛细胞有类似的模式，但GAD+末梢的簇排列没那么紧密。星状细胞上的反应性末梢分布更均匀。章鱼细胞上的GAD+末梢比毛细胞上的更大（直径可达2.5微米），并且倾向于在胞体和树突上聚集成小簇或排成行。反应性末梢含有小的多形性囊泡，并在所检查的每种细胞类型上形成对称的突触接触。每种神经元类型上由GAD+末梢形成的模式类似于先前用高尔基方法描述的某些类型的非耳蜗轴突的模式，这些轴突从背侧耳蜗核和梯形体投射而来。
